C7898

Concanavalin A from Canavalia ensiformis (Jack bean)

ferritin conjugate, Type IV, buffered aqueous glycerol solution

• Synonym(s): ConA
• UNSPSC Code: 12352200

Properties

• conjugate: ferritin conjugate
• type: Type IV
• form: buffered aqueous glycerol solution
• concentration: 5-10 mg/mL protein (Biuret)
• extent of labeling: ~1 mol ferritin per mol lectin
• impurities: carbohydrate, essentially free
• storage temp.: −20°C

Description

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Packaging

Package size based on protein content

Physical form

Solution in 50% glycerol containing 0.3 M NaCl, 0.01 M sodium phosphate, 0.06 M CaCl₂, 0.06 mM MnCl₂ and 0.02% sodium azide as preservative

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.