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C5223

Sigma-Aldrich

Anti-Cdk2 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous glycerol solution

Synonym(s):

Anti-CDKN2, Anti-p33(CDK2)

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About This Item

MDL number:
MFCD00675227
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

rat (predicted), human, mouse (predicted)

technique(s)

immunoprecipitation (IP): 2-4 μg using 500 μg of HeLa cell nuclear extract
western blot: 0.5-2 μg/mL using human HeLa cell nuclear extract

UniProt accession no.

P24941

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CDK2(1017)
mouse ... Cdk2(12566)

Specificity

The antibody reacts specifically with human cyclin-dependent kinase 2 (Cdk2) (p33cdk2).

Immunogen

synthetic peptide corresponding to amino acids 287-298 (CQDVTKPVPHLRL) of human cdk2.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunoprecipitation (1 paper)

Physical form

Solution in 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, and 0.05% sodium azide containing 30% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Christoffer Bento et al.
BMC cell biology, 10, 89-89 (2009-12-19)
The DDIT3 gene encodes a transcription factor belonging to the CCAAT/enhancer binding protein (C/EBP) family. It is normally expressed at very low levels but is activated by cellular stress conditions and induces G1 arrest and, in some cell types, apoptosis.

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