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C4743

Sigma-Aldrich

Monoclonal Anti-Cytochrome P450 3A5 antibody produced in mouse

~2 mg/mL, clone F18 P3 B6, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-CP35, Anti-CYP3A5, Anti-P450PCN3, Anti-PCN3

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About This Item

UNSPSC Code:
12352203

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

F18 P3 B6, monoclonal

form

buffered aqueous solution

mol wt

antigen ~55 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.5-1.0 μg/mL using human recombinant cytochrome P450 3A5.

isotype

IgG1

UniProt accession no.

P20815

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CYP3A5(1577)

Specificity

The antibody does not cross react with cytochrome P450 3A4 or 3A7.

Immunogen

synthetic peptide corresponding to amino acids 380-389 of human cytochrome P450 3A5.

Application

Monoclonal Anti-Cytochrome P450 3A5 antibody produced in mouse is suitable for:
  • immunohistochemistry
  • indirect ELISA
  • western blotting at a concentration of 0.5-1.0 μg/mL using human recombinant cytochrome P450 3A5

Biochem/physiol Actions

Cytochrome P450 enzymes are a super family of heme containing mono-oxygenases that in humans are involved with oxidative metabolism of xenobiotics. This metabolism is the initial step in the biotransformation and elimination of a wide variety of drugs and environmental pollutants from the body. The Cytochrome P450 3A (CYP 3A) family contains several isoforms with the CYP 3A4 isoform contributing most of the family activity and the CYP 3A5, 3A7 and 3A43 being the minor isoforms.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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P Anzenbacher et al.
Cellular and molecular life sciences : CMLS, 58(5-6), 737-747 (2001-07-05)
Cytochromes P450 (henceforth P450s) are involved in a variety of metabolic and biosynthetic processes. The number of known P450 enzymes exceeds 1000, while the endogenous substrates of most of them remain unknown. All P450 enzymes exhibit similarity in their structure
Meera Kumarakulasingham et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 11(10), 3758-3765 (2005-05-18)
The cytochromes P450 (P450) are a multigene family of enzymes with a central role in the oxidative metabolism of a wide range of xenobiotics, including anticancer drugs, carcinogens, and endogenous compounds. The purpose of this study was to define the

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