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A9264

Sigma-Aldrich

Adenosine 5′-triphosphate–Agarose

lyophilized powder

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About This Item

MDL number:
UNSPSC Code:
41106514

form

lyophilized powder

extent of labeling

1-5 μmol per mL

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

N-6

matrix spacer

11 atoms

capacity

0.5-2.5 μmol/mL (ATP)

storage temp.

−20°C

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Application

Adenosine 5′-triphosphate Agarose (5′-ATP agarose) has been used in affinity chromatography to purify uridine kinase from Ehrlich ascites tumor cells.

Physical form

Lyophilized powder stabilized with lactose

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Joanna F Swain et al.
The Journal of biological chemistry, 281(3), 1605-1611 (2005-11-09)
The Hsp70 family of molecular chaperones acts to prevent protein misfolding, import proteins into organelles, unravel protein aggregates, and enhance cell survival under stress conditions. These activities are all mediated by recognition of diverse hydrophobic sequences via a C-terminal substrate-binding
R Scaife et al.
The Journal of cell biology, 111(6 Pt 2), 3023-3033 (1990-12-01)
We have purified a 100-kD rat brain protein that has microtubule cross-linking activity in vitro, and have determined that it is dynamin, a putative microtubule-associated motility protein. We find that dynamin appears to be specific to neuronal tissue where it
Purification and characterization of acetate kinase from <I>Clostridium thermocellum</I>.
Lin, W.R., et al.
Tetrahedron, 54, 15915-15925 (1998)
Yasuo Nakahara et al.
Journal of basic microbiology, 44(6), 459-470 (2004-11-24)
UDPgalactose:polysaccharide galactosyl-transferase is the enzyme that is specifically localized in prespore cells of Dictyostelium discoideum and its activity sharply changes in response to differentiation and dedifferentiation. To clarify the nature of this enzyme, we first developed an improved assay method
Katarzyna Banaszak et al.
Journal of molecular biology, 407(2), 284-297 (2011-01-19)
Phosphofructokinase 1 (PFK) is a multisubunit allosteric enzyme that catalyzes the principal regulatory step in glycolysis-the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate by ATP. The activity of eukaryotic PFK is modulated by a number of effectors in response to

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