A5838
Anti-Glutathione-S-Transferase (GST)–Alkaline Phosphatase Conjugate antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-Glutathione-S-Transferase, Anti-GST
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
rabbit
conjugate
alkaline phosphatase conjugate
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 27.5 kDa
technique(s)
direct ELISA: 1:400 using using wells coated with 5 μg/mL GST in carbonate buffer
western blot (chemiluminescent): 1:20,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
Recombinant target proteins are often expressed as a fusion product with Glutathione-S-Transferase (GST) tags using various expression vector constructs. Thus, antibodies directed against the GST tags of the recombinant constructs can facilitate the purification and study of target proteins.
The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.
Immunogen
recombinant GST from Schistosoma japonicum expressed in E. coli.
Application
Alkaline phosphatase (AP) conjugated anti-GST antibodies have been used as probes for western blot assays. The product can also be used for direct ELISA (1:400).
Purification of GST-SfMannIII from Sf9 insect cells was confirmed by western blot using rabbit anti-GST antibody. Gst-SfMan I was purified from over-expressing SF/SWT-1 cells and ran on SDS-page fell for western blot analysis using rabbit anti-GST as the primary antibody. Alkaline phosphatase-conjugated goat anti-rabbit IgG (Sigma) was used as the secondary antibody.
Physical form
Solution supplied in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% BSA, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Z Kawar et al.
The Journal of biological chemistry, 276(19), 16335-16340 (2001-03-30)
Previously, we cloned and characterized an insect (Sf9) cell cDNA encoding a class II alpha-mannosidase with amino acid sequence and biochemical similarities to mammalian Golgi alpha-mannosidase II. Since then, it has been demonstrated that other mammalian class II alpha-mannosidases can
Jared J Aumiller et al.
Glycobiology, 13(6), 497-507 (2003-03-11)
We have previously engineered transgenic insect cell lines to express mammalian glycosyltransferases and showed that these cells can sialylate N-glycoproteins, despite the fact that they have little intracellular sialic acid and no detectable CMP-sialic acid. In the accompanying study, we
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T-box factors Tbx2 and Tbx3 play key roles in the development of the cardiac conduction system, atrioventricular canal, and outflow tract of the heart. They regulate the gap-junction-encoding gene Connexin43 (Cx43) and other genes critical for heart development and function.
Qian-Kun Niu et al.
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Pollen germination and embryogenesis are important to sexual plant reproduction. The processes require a large number of genes to be expressed. Transcription of eukaryotic nuclear genes is accomplished by three conserved RNA polymerases acting in association with a set of
Zhao-Xia Ma et al.
PloS one, 10(7), e0132500-e0132500 (2015-07-18)
The Arabidopsis TMS1 encodes a heat shock protein identical to the Hsp40 protein AtERdj3A and plays important roles in the thermotolerance of pollen tubes and other plant tissues. Despite its importance to plant growth and reproduction, little has been known
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