A4721

Monoclonal Anti-ARNO (Cytohesin-2) antibody produced in mouse

~2 mg/mL, clone CYT2-21, purified immunoglobulin, buffered aqueous solution

• MDL number: MFCD03454755
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: CYT2-21, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 40 kDa
• species reactivity: rat, human
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 5-10 μg/mL using a whole extract of rat brain
• isotype: IgG1
• UniProt accession no.: Q99418
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CYTH2(9266) ; rat ... Cyth2(116692)

Description

General description

ARNO (Cytohesin-2) is a guanine-nucleotide exchange factor that belongs to Arf family of GTP-associated proteins. ARNO has been shown to modulate the movement of mouse preadipocyte 3T3-L1 cells. Studies have reported that cytohesin-2 is overexpressed in pulmonary cancers and can also serve as biomarker of prognosis in hepatocellular carcinomas.
Monoclonal Anti-ARNO (Cytohesin-2) reacts specifically with ARNO, and does not cross-react with other members of the cytohesin family. The antibody associates with human and rat ARNO.

Immunogen

recombinant, human ARNO (ARFnucleotide-binding-site opener).

Application

Mouse monoclonal anti-ARNO (Cytohesin-2) antibody can be used for western blot (5-10μg/mL using a whole extract of rat brain), microarray, indirect ELISA and immunocytochemistry applications.

To determine if ARNO binds to phosphoinositides, a protein-overlay assay was performed using monoclonal mouse anti-ARNO as the primary antibody for ARNO detection on membranes spotted with phosphoinositides and incubated with ARNO.[1]

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.