A2602

Asn-Arg-Cys-Ser-Gln-Gly-Ser-Cys-Trp-Asn, Reduced

≥85% (HPLC)

• Empirical Formula (Hill Notation): C₄₄H₆₇N₁₇O₁₆S₂
• Molecular Weight: 1154.24
• MDL number: MFCD00798787
• UNSPSC Code: 12352200
• PubChem Substance ID: 329770789

Properties

• assay: ≥85% (HPLC)
• form: powder
• technique(s): ligand binding assay: suitable
• color: white
• storage temp.: −20°C
• SMILES string: N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CS)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CC(N)=O)C(O)=O
• InChI: 1S/C44H67N17O16S2/c45-21(11-32(47)65)35(68)55-23(6-3-9-51-44(49)50)37(70)60-30(18-79)42(75)59-28(16-63)40(73)56-24(7-8-31(46)64)36(69)53-14-34(67)54-27(15-62)39(72)61-29(17-78)41(74)57-25(38(71)58-26(43(76)77)12-33(48)66)10-19-13-52-22-5-2-1-4-20(19)22/h1-2,4-5,13,21,23-30,52,62-63,78-79H,3,6-12,14-18,45H2,(H2,46,64)(H2,47,65)(H2,48,66)(H,53,69)(H,54,67)(H,55,68)(H,56,73)(H,57,74)(H,58,71)(H,59,75)(H,60,70)(H,61,72)(H,76,77)(H4,49,50,51)/t21-,23-,24-,25-,26-,27-,28-,29-,30-/m0/s1
• InChI key: KEQZTNKTLNAKCM-XFPWREGGSA-N

Description

Amino Acid Sequence

Asn-Arg-Cys-Ser-Gln-Gly-Ser-Cys-Trp-Asn

Biochem/physiol Actions

The activity of the enzymes protein disulfide-isomerase (PDI) and DsbA (a periplasmic protein thiol:disulfide oxidoreductase) can be monitored with this peptide. Oxidation of this dithiol peptide to the disulfide state is accompanied by a significant change in tryptophan fluorescence emission intensity. This permits the rapid determination of the pH-dependence of the activity of both enzymes.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)