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A0157

Sigma-Aldrich

Ascorbate Oxidase from Cucurbita sp.

lyophilized powder, 1,000-3,000 units/mg protein

Synonym(s):

L-Ascorbate:oxygen oxidoreductase, Ascorbase

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100 UNITS
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250 UNITS
$123.00
1000 UNITS
$320.15

$61.00

Available to ship onApril 28, 2025Details

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100 UNITS
$61.00
250 UNITS
$123.00
1000 UNITS
$320.15

About This Item

CAS Number:
9029-44-1
Enzyme Commission number:
1.10.3.3 (BRENDA, IUBMB)
EC Number:
232-852-6
MDL number:
MFCD00130560
UNSPSC Code:
12352204
NACRES:
NA.54

$61.00

Available to ship onApril 28, 2025Details

biological source

plant (Cucurbita spp.)

Quality Level

200

form

lyophilized powder

specific activity

1,000-3,000 units/mg protein

storage temp.

2-8°C

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General description

Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70kDa per subunit.[1] This enzyme is mainly found in plants, fungi and eubacteria.[2]

Application

Ascorbate Oxidase from Cucurbita sp. has been used:
  • as a supplement in the culture medium for differentiation into osteoblasts[3]
  • to oxidize ascorbic acid producing monodehydroascorbate in monodehydroascorbate reductase assay[4]
  • in determining ascorbate (AsA) and dehydroascorbate (DHA) concentrations[5]

Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Ascorbate oxidase, from Sigma, has been used in ascorbic acid assays to study the heat stress response of Arabidopsis [6].

Biochem/physiol Actions

Ascorbate oxidase (AO) participates in cell growth by regulating reduction/oxidation (redox) of the apoplast. This enzyme can synthesize the oxidative molecule dehydroascorbate acid (DHA) in the apoplast. It is also involved in cell elongation and enlargement development.[5]
Ascorbate oxidase converts ascorbic acid to dehydroascorbic acid. It is highly specific for L-ascorbic acid and a few analogs. Ascorbate oxidase exists as a dimer and has a molecular weight of approximately 140 kDa.

Unit Definition

One unit will oxidize 1.0 μmole of L-ascorbate to dehydroascorbate per min at pH 5.6 at 25 °C.

Physical form

Lyophilized powder containing buffers and sucrose as stabilizer.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000335079
0000335081
0000335078
0000335081
0000335079

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Manuel A Matamoros et al.
Journal of experimental botany, 61(1), 87-97 (2009-10-14)
In plant cells, antioxidants keep reactive oxygen species at low concentrations, avoiding oxidative damage while allowing them to play crucial functions in signal transduction. However, little is known about the role of antioxidants during fruit maturation, especially in legumes. Snap
Cotton ascorbate oxidase promotes cell growth in cultured tobacco bright yellow-2 cells through generation of apoplast oxidation
Li R, et al.
International Journal of Molecular Sciences, 18(7), 1346-1346 (2017)
Ming Liu et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 27(5), 477-477 (2011-05-12)
An amperometric L-ascorbic acid (AA) biosensor fabricated by immobilizing ascorbate oxidase (AO) in poly(3,4-ethylenedioxythiophene) (PEDOT) and multi-walled carbon nanotubes (MWCNTs) composite films was reported for the first time. The entrapment of AO in PEDOT/MWCNTs composite films was performed during an
Copper metalloenzymes
Messerschmidt A
Comprehensive Natural Products II: Chemistry and Biology (2010)
Ethylene response factor 6 is a regulator of reactive oxygen species signaling in Arabidopsis
Sewelam N, et al.
PLoS ONE, 8(8), e70289-e70289 (2013)
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