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U0880

Sigma-Aldrich

Uricase from Candida sp.

Uricase from Candida sp.

recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid

Synonym(s):

Urate: oxygen oxoreductase

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250 UNITS
$172.00
1000 UNITS
$511.00

$172.00

Estimated to ship onFebruary 17, 2025

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250 UNITS
$172.00
1000 UNITS
$511.00

About This Item

CAS Number:
9002-12-4
Enzyme Commission number:
1.7.3.3 (BRENDA, IUBMB)
MDL number:
MFCD00082126
UNSPSC Code:
12352204
PubChem Substance ID:
24900619
NACRES:
NA.54

$172.00

Estimated to ship onFebruary 17, 2025

Request a Bulk Order

recombinant

expressed in E. coli

Quality Level

200

form

lyophilized powder

specific activity

≥2 units/mg solid

mol wt

35 kDa by SDS-PAGE

storage temp.

−20°C

SMILES string

[P](=O)([O-])(OC3C(OC(C3O)N4C=CC(=O)NC4=O)CO)OCC1OC(C(C1O)O)N2C=CC(=O)NC2=O.[N+H4]

InChI

1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3

InChI key

VPXDHZMLJOJKOX-UHFFFAOYSA-N

General description

Uricase deficient mice develop uric acid nephropathy and diabetes insipidus which can be mitigated with recombinant uricase treatment. [1]
Uricase is a homo-tetrameric protein with its active sites at the head-to-tail monomer-dimer interface.

Application

Uricase from Candida sp. has been used:
  • to eliminate urate in plasma samples and evaluate the contribution of urate to the total plasma antioxidant capacity
  • to intraperitoneally inject the mice to study the role of uric acid in fibrin extracellular traps (ET) formation
  • for elimination of uric acid in ferric reducing/antioxidative power (FRAP) assay to measure the total antioxidative capacity of human plasma sample; in uricase-based biosensors

Biochem/physiol Actions

Uricase participates in purine catabolism. It catalyzes the conversion of highly insoluble uric acid into 5-hydroxyisourate. Accumulation of uric acid causes liver/kidney damage or chronically causes gout. In mice, a mutation in the gene encoding uricase causes a sudden increase in uric acid. Mice, deficient in this gene, exhibit hyperuricemia, hyperuricosuria, and uric acid crystalline obstructive nephropathy.

Unit Definition

One unit will oxidize 1.0 μmole of uric acid to allantoin per minute at pH 8.5 at 25 °C.

Physical form

Powder; contains citrate and sucrose

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000380939
0000349793
0000327059
0000380939
0000327059

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Darko Duplancic et al.
Molecules (Basel, Switzerland), 16(8), 7058-7068 (2011-08-19)
Determination of the relative contribution of uric acid level increases to the total measured antioxidative activity could be very useful for testing antioxidative products and their effect on human health. The aim of this report is to present a simple
Rocco Cancelliere et al.
Biosensors, 12(1) (2022-01-21)
The use of carbon nanomaterials (CNMs) in sensors and biosensor realization is one of the hottest topics today in analytical chemistry. In this work, a comparative in-depth study, exploiting different nanomaterial (MWNT-CO2H, -NH2, -OH and GNP) modified screen-printed electrodes (SPEs)
Benoit Pugin et al.
Frontiers in microbiology, 13, 853735-853735 (2022-05-03)
Uricase catalyzes the conversion of uric acid into allantoin with concomitant reduction of molecular oxygen to hydrogen peroxide. In humans, uricase is not functional, thereby predisposing individuals to hyperuricemia, a metabolic disturbance associated with gout, chronic kidney disorders, and cardiovascular
Darko Modun et al.
Atherosclerosis, 197(1), 250-256 (2007-05-15)
By using red wine, dealcoholized red wine, polyphenols-stripped red wine, ethanol-water solution and water, the role of wine polyphenols and induction of plasma urate elevation on plasma antioxidant capacity was examined in humans (n=9 per beverage). Healthy males randomly consumed
Michael W Munks et al.
Blood, 116(24), 5191-5199 (2010-09-30)
It has been recognized for nearly 80 years that insoluble aluminum salts are good immunologic adjuvants and that they form long-lived nodules in vivo. Nodule formation has long been presumed to be central for adjuvant activity by providing an antigen
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Questions

1–2 of 2 Questions  

  1. How can a solution in unit/mL be prepared using product number U0880, with a pack size of 250 units and SKU U0880-250UN, considering that the uricase activity is greater than 2 units/mg solid? What is the procedure for preparing a 10 unit/mL solution from this?

    1 answer

    1. You can use the calculator provided at https://www.physiologyweb.com/calculators/units_per_volume_solution_concentration_calculator.html

      Based on a specific activity of 5 units/mg, the 250 unit pack size is equivalent to 50 mg in mass. To achieve a 10 units/mL solution from this, the 50 mg would need to be dissolved in 25 mL of solvent (e.g. PBS).

      Helpful?

  2. What is the amino acid sequence of this enzyme?

    1 answer

    1. The sequence is proprietary.

      Helpful?

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