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89827

Sigma-Aldrich

Fluorescent IEF-Marker pI 4.0 solution

1 mg/mL in H2O

Synonym(s):

IEF-Marker fluorescent pI 4.0

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About This Item

MDL number:
MFCD03099322
UNSPSC Code:
12352200
NACRES:
NA.26

mol wt

Mw 250.29 g/mol

concentration

1 mg/mL in H2O

fluorescence

λex 310 nm; λem 415 nm in 0.1 M citrate pH 4.0

application(s)

cleaning products
cosmetics
flavors and fragrances
food and beverages
personal care

storage temp.

2-8°C

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General description

filtered through a 0.45 μm membrane filter

Application

Suitable as IEF-Marker for HPCE and for IEF-gels

Reconstitution

For use in HPCE the marker should be diluted 1:100 in a suitable ampholyte solution (e.g. 2% ampholyte 3-10), UV-detection at 280 nm (20 °C). For use in IEF-gels the solution can be loaded directly onto the gel (max. 1μl per lane).

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

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Minh Khang Chau et al.
Analytica chimica acta, 1124, 60-70 (2020-06-15)
We report capacitively coupled contactless conductivity detection (C4D) of proteins separated by microfluidic capillary isoelectric focusing (μCIEF). To elucidate the evolution of negative conductivity peaks during focusing and seek IEF conditions for sensitive conductivity detection, numerical simulation was performed using

Articles

Isoelectric Focusing

Information on Isoelectric Focusing including what it is and how it is used. In order to ensure the high performance of analysis, isoelectric point (pI) standards are needed.

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