The 96992-3000TESTS vial will include 30 milliliters of reagent.
96992
Cell Counting Kit - 8
for quantitation of viable cell number in proliferation and cytotoxicity assays
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100 TESTS
$95.40
500 TEST
$386.00
3000 TEST
$1,940.00
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100 TESTS
$95.40
500 TEST
$386.00
3000 TEST
$1,940.00
About This Item
UNSPSC Code:
12352108
NACRES:
NA.32
Recommended Products
Quality Level
shelf life
limited shelf life, expiry date on the label
shipped in
ambient
storage temp.
2-8°C
Application
Cell Counting Kit-8 (CCK-8) allows convenient assays using WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt), which produces a water-soluble formazan dye upon bioreduction in the presence of an electron carrier, 1-Methoxy PMS. CCK-8 solution is added directly to the cells, no pre-mixing of components is required. WST-8 is bioreduced by cellular dehydrogenases to an orange formazan product that is soluble in tissue culture medium. The amount of formazan produced is directly proportional to the number of living cells. Since the CCK-8 solution is very stable and it has little cytotoxicity, a longer incubation, such as 24 to 48 hours, is possible.
Cell Counting Kit-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, XTT or MTS.
Cell Counting Kit-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, XTT or MTS.
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Product No.
Description
Pricing
signalword
Warning
hcodes
Hazard Classifications
Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Articles
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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What is the solution volume provided in the "3000 tests"?
1 answer-
Helpful?
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100 Test means 100 wells, so only one 96-well plate?
1 answer-
The reagents provided in kit 96992 are sufficient for 100 tests. A 96 well microplate is not included with the kit. The customer must provide the 96 well plate. Although a solid 96 well plate can be used, a 96 well microplate with removable strips will allow for the most efficient use of the kit.
Helpful?
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How much volume (in milliliters) is present in each vial of the CCK-8 solution?
1 answer-
The volume supplied for Product No. 96992, Cell Counting Kit, is as follows:
•100 tests: 1 ml x 1 vial
•500 tests: 5 ml x 1 vial
•3000 tests: 5 ml x 6 vialsHelpful?
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Is this kit suitable for use with HEK293 or HEK293f cells?
1 answer-
Yes, the 96992 kit can be utilized to stain both HEK293 and HEK293B cells. The Cell Count kit is not cell type-specific and is expected to function effectively for counting viable mammalian cells. In this kit, the WST-8 dye, which is a formazan stain is specific to viable cells and is not present in nonviable cells.
Helpful?
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What are the volumes of reagents included in the 96992-100test-F kit?
1 answer-
Supplier information specifies that the kit contains 6 bottles, each containing 5 ml of solution. Based on the product datasheet, each well of a well-plate requires 10 microliters of solution, indicating that for 3000 tests, the kit should contain 30 ml of solution. Using these calculations, for the 100 test kit, each bottle would contain approximately 167 microliters of solution (5 ml divided by 30 = 167 microliters).
Helpful?
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Hi, what is the solution volume provided in the "500 tests" SKU? Thanks in advance, Aviv.
1 answer-
The 500 test volume is 5 mL. See the links below to the Product datasheet and the Product Troubleshooting Guide for additional information.
Product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/381/017/96992dat.pdfTroubleshooting Guide:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/129/020/96992-product-information.pdfHelpful?
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