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64010

Sigma-Aldrich

Metanil Yellow

suitable for microscopy

Synonym(s):

3-(4-Anilinophenylazo)benzenesulfonic acid sodium salt, Acid Yellow 36

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About This Item

Empirical Formula (Hill Notation):
C18H14N3NaO3S
CAS Number:
Molecular Weight:
375.38
Colour Index Number:
13065
Beilstein/REAXYS Number:
3831568
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

form

powder

solubility

methanol: 0.01 g/10 mL, orange to very deep orange

εmax

≥500 at 409-419  nm in methanol

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[O-]S(=O)(=O)c1cccc(c1)\N=N\c2ccc(Nc3ccccc3)cc2

InChI

1S/C18H15N3O3S.Na/c22-25(23,24)18-8-4-7-17(13-18)21-20-16-11-9-15(10-12-16)19-14-5-2-1-3-6-14;/h1-13,19H,(H,22,23,24);/q;+1/p-1/b21-20+;

InChI key

NYGZLYXAPMMJTE-ANVLNOONSA-M

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Application

Metanil yellow has been used for staining of leukocyte granules.[1] It is also used as a counterstain in microscopic sections.[2]

Biochem/physiol Actions

Metanil yellow is an azo dye.[3] It is used as a counterstain in PAS (periodic acid-Schiff)/iron-hematoxylin stained sections to increase contrast.[4]

pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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An improved stain for connective tissue cells in plastic-embedded sections.
Laboratory Medicine, 10, 783-785 (1979)
Photocatalytic degradation of azo dye Metanil Yellow: optimization and kinetic modeling using a chemometric approach.
Applied Catalysis. B, Environmental, 77, 1-11 (2007)
Enhancement of histological detail using metanil yellow as counterstain in periodic acid Schiff's hematoxylin staining of glycol methacrylate tissue sections.
Quintero-Hunter I, et al.
Biotechnic & Histochemistry, 66, 169-169 (1991)
Cellular localization of latent murine cytomegalovirus.
Koffron AJ, et al.
Journal of Virology, 72, 95-95 (1998)
P B Rastogi et al.
Journal of food protection, 59(3), 244-248 (1996-03-01)
Among a variety of experimental protocols used, the combined use of 0.5% dextrose in bottom agar and 1 micromol of flavin mononucleotide (FMN) in preincubation mixtures without fraction S9 mix resulted in the highest level of induced Salmonella typhimurium his

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