03285
Cell Counting Kit
sufficient for 500 tests
Synonym(s):
Fluorescent Cell Counter
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About This Item
UNSPSC Code:
12352116
eCl@ss:
32161000
NACRES:
NA.32
Recommended Products
form
liquid
usage
sufficient for 500 tests
storage temp.
−20°C
General description
Cell Counting kit, also known as FCCK, is a fluorescent cell counting product. The amount of a fluorescent dye, calcein, produced from Calcein-AM (3′,6′-Di(O-acetyl)-2′,7′-bis [N, Nbis-(carboxymethyl) aminomethyl]-fluorescein, tetraacetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Calcein-AM is highly lipophilic due to the presence of acetoxymethyl groups in the molecule, which rapidly permeates into the cell cytoplasm through the membrane.
Application
Cell Counting Kit or FCCK is used for the detection of live cells by fluorometric method.
Features and Benefits
Cell Counting Kit has the following advantages:
- It doesn’t require any radioisotopes to function.
- No solubilization procedure is needed.
- Results are highly reproducible and accurate.
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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