PPB010

Tris EDTA Buffer, pH 8.0

pHast Pack^™, powder, 10X concentrate

• Synonym(s): 10X TE buffer
• UNSPSC Code: 12161700
• NACRES: NA.25

Properties

• product line: pHast Pack^™
• form: powder
• storage condition: (Tightly closed. Dry)
• impurities: ≤5 ppm Heavy Metals (lead)
• color: white
• pH: 7.9—8.1
• solubility: soluble, clear, colorless
• cation traces: Fe: <10 ppm
• foreign activity: DNAse, none detected; Nickase, none detected; Protease, none detected; RNAse, none detected
• storage temp.: room temp

Description

General description

10X Tris-EDTA or TE buffer has two components. Tris buffer is a commonly used stable, non-toxic, and non-hygroscopic buffer. Also, ethylenediaminetetraacetic acid (EDTA), a metal chelator, binds with cation metal ions that further inactivate the DNase enzyme which requires metal ions to activate. It has a pH range of 7.9 - 8.1.

Application

TE buffer is intended for use in:

• Nucleic acid gel electrophoresis
• storage and dilution of DNA and RNA
• DNA automated sequencing
• Immunohistochemistry

Features and Benefits

• Fast and easy Ready-to-use buffer
• Minimizes effort - no need to weigh and adjust pH
• Saves time and eliminates exposure to hazardous chemicals
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Packaging

Foil pouch

Reconstitution

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 10X solution containing 100mM Tris, 10mM EDTA, pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• pictograms: GHS08
• signalword: Warning
• hcodes: H373
• pcodes: P260 - P314 - P501
• Hazard Classifications: STOT RE 2 Inhalation
• target_organs: Respiratory Tract
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable