SRE0062
Tris-Borate EDTA Buffer, 10X Concentrate
Synonym(s):
TBE Buffer
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About This Item
UNSPSC Code:
41105319
NACRES:
NA.25
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Application
Tris-Borate EDTA (TBE) buffer is commonly used in the electrophoresis of nucleic acids in agarose and polyacrylamide gels.
TBE buffer is recommended for resolution of RNA and DNA fragments smaller than 1500 bp.
TBE buffer is recommended for resolution of RNA and DNA fragments smaller than 1500 bp.
Physical form
10X TBE buffer stock solution contains 0.89 M Trizma(R) with 0.02 M EDTA adjusted to pH 8.3 with boric acid. Dilute with ultrapure water to 1X.
Notes: If precipitation is present in the 10X concentrated buffer, warm the bottle to 37 °C and mix until completely dissolved prior to dilution.
It is recommended 1X working solutions be filtered through a 0.2 mm filter before use.
Notes: If precipitation is present in the 10X concentrated buffer, warm the bottle to 37 °C and mix until completely dissolved prior to dilution.
It is recommended 1X working solutions be filtered through a 0.2 mm filter before use.
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Danger
hcodes
Hazard Classifications
Repr. 1B
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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