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30503

Sigma-Aldrich

Phenolic auramine solution

suitable for microscopy

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About This Item

UNSPSC Code:
12171500
NACRES:
MA.02

shelf life

limited shelf life, expiry date on the label

Quality Level

suitability

suitable for microscopy

storage temp.

2-8°C

General description

The Auramine is part of Flourecent Stain Kit for Mycobacteria (Cat. No. 05151). It is used to stain acid-fast organisms as well as Mycobacterium sp. in specimens and in culture. This flourescent method, actually the best procedure, stains selectively mycobacteria by binding dye to the mycolic acid of the cell wall.

application

Phenolic auramine solution staining can be used for sputum smear microscopy as well as fluorescence microscopy, for diagnosing sputum-smear-negative pulmonary tuberculosis (PTB), in human immunodeficiency virus (HIV) positive population, especially in sub-Saharan Africa region.[1]

Components

Composition
Auramine O 3.0 g/l, Phenol 30.0 g/l, in water

pictograms

Health hazardCorrosion

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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An approach to the problems of diagnosing and treating adult smear-negative pulmonary tuberculosis in high-HIV-prevalence settings in sub-Saharan Africa.
Harries AD
Bulletin of the World Health Organization, 76 (6), 651-651 (1998)

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