Skip to Content
MilliporeSigma
All Photos(1)

Documents

TRIPURE-RO

Roche

TriPure™ Isolation Reagent

solution (ready-to-use), pkg of 50 mL (11667157001), pkg of 200 mL (11667165001)

Synonym(s):

DNA purification, Protein isolation, RNA isolation, nucleic acid isolation

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41105500

form

solution (ready-to-use)

Quality Level

100

packaging

pkg of 200 mL (11667165001)
pkg of 50 mL (11667157001)

manufacturer/tradename

Roche

storage temp.

2-8°C

General description

Monophasic solution of phenol and guanidine thiocyanate for RNA, DNA, and protein isolation.

Application

Ready-to-use reagent for the simultaneous isolation of total RNA, DNA, and proteins from cells or tissues of human, plant, yeast, bacterial, or viral origin. RNA is undegraded and free of protein or DNA contamination according to the current Quality Control.After purification, many applications are possible:
  • The DNA-free total RNA is suitable for northern blotting, in vitro translation, RNase protection assays, cDNA synthesis, or RT-PCR.
  • The RNA-free DNA is suitable for PCR, restriction digestion, Southern blotting, or cloning.
  • The denatured protein is suitable for SDS-PAGE and western blotting.

Features and Benefits

The TriPure Isolation Reagent allows the simultaneous isolation of RNA, DNA, and protein from the same sample in a single-step liquid-phase separation. RNA, DNA, and protein are separated into different organic and aqueous phases, from which each can be purified by a series of alcohol precipitations.
  • Work with an easy-to-use kit. The red dye in the reagent simplifies identification of different phases.
  • Process a wide variety of starting samples. Easily adapt the kit to your specific needs.
  • Simplify isolation protocols. Use a single reagent that isolates DNA-free RNA, RNA-free DNA, and proteins.
  • Increase yields of intact RNA. TriPure Isolation Reagent provides an immediate chaotropic denaturing environment that eliminates endogenous RNase activity.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

TriPure is a trademark of Roche

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

supp_hazards

EUH032 - EUH071

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

JanWillem Duitman et al.
Laboratory investigation; a journal of technical methods and pathology, 94(1), 89-97 (2013-11-20)
CCAAT-enhancer-binding protein delta (C/EBPδ) is a transcription factor mainly known for its role in inflammation and apoptosis/proliferation. Considering that these are key processes in renal fibrosis, we hypothesized that C/EBPδ would potentiate renal fibrosis. In line with this hypothesis, C/EBPδ
Melike Dinccelik-Aslan et al.
Molecular and clinical oncology, 3(3), 584-590 (2015-07-03)
Gastric Cancer is one of the most common malignancies worldwide and the second most common cause of cancer-related mortality. Previous studies revealed several genetic alterations specific to gastric cancer. In this study, we aimed to investigate the diagnostic and prognostic
Hou-Zao Chen et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(36), 11897-11912 (2014-09-05)
The failure of past efforts to develop effective stroke treatments is at least partially because these treatments often interfered with essential physiological functions, even though they are targeted toward pathophysiological events, such as inflammation, excitotoxicity, and oxidative stress. Thus, the

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service