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Sigma-Aldrich

TRI Reagent®

for DNA, RNA and protein isolation

Synonym(s):

DNA/RNA/protein extraction reagent, DNA/RNA/protein isolation reagent, DNA/RNA/protein purification reagent, single step RNA extraction reagent, total RNA extraction reagent, TRI Reagent® RNA Isolation Reagent

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About This Item

MDL number:
MFCD00213058
UNSPSC Code:
12352200
NACRES:
NA.52

grade

for DNA, RNA and protein isolation

form

liquid

usage

 mL sufficient for 107 cells
 mL sufficient for 100 mg tissue

impurities

phenol

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General description

TRI reagent® also known as TRIzol, is a monophasic solution of phenol and guanidinium isothiocyanate. It has a potential, to simultaneously solubilize biological material and denatures protein. It is widely used for deproteinizing RNA. This improved version of the single-step total RNA isolation reagent developed by Chomczynski allows a quick, economical, and efficient isolation of total RNA from different biological samples. This product is pure (A260/A280 ~1.8) and can be directly used in Northern blotting. Simultaneous isolation of DNA, RNA and proteins is also possible. 1 mL is sufficient for the extraction of 100 mg tissue or 107 cells.

Application

TRI Reagent® has been used for isolating RNA from various types of cells or tissues. It may also be used for processing tissues, cells cultured in monolayer, or cell pellets.
TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.

Features and Benefits

  • Easily scalable RNA isolation
  • Works with many sources: human, plant, yeast, bacterial, or viral
  • Better yields than traditional guanidine thiocyanate/cesium chloride methods

Legal Information

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

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Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

target_organs

Nervous system,Kidney,Liver,Skin

supp_hazards

EUH032

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

174.2 °F - closed cup

flash_point_c

79 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

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Expression of adenosine receptors and vegf during angiogenesis and its inhibition by pentoxifylline?a study using zebrafish model
Nathan J, et al.
Biomedicine and Pharmacotherapy, 84(6), 1406-1418 (2016)
Hao Zhang et al.
Molecular medicine reports, 22(2), 915-925 (2020-07-07)
Dilated cardiomyopathy (DCM) is a primary cardiomyopathy with high mortality. The aim of the present study was to identify the related genes in DCM. The four expression profiles (GSE17800, GSE21610, GSE42955 and GSE79962) downloaded from the Gene Expression Omnibus (GEO)
Purification of RNA using TRIzol (TRI reagent)
Rio D, et al.
Cold Spring Harbor Protocols, 2010(6), pdb-prot5439 (2010)
Ruochan Chen et al.
Science advances, 5(5), eaav5562-eaav5562 (2019-05-28)
The ability of cytosolic lipopolysaccharide (LPS) to activate caspase-11-dependent nonclassical inflammasome is intricately controlled to avoid excessive inflammatory responses. However, very little is known about the regulatory role of various metabolic pathways in the control of caspase-11 activation. Here, we
Hybrid amniotic membrane dressing with green silver nanoparticles as bioengineered skin for wounds and burns: a pilot studies
Ramesh B, et al.
Journal of biotechnology and biomaterials, 7(272), 2-2 (2017)
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Articles

Genomic DNA Purification and Assessment

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

Protocols

TRI Reagent® Protocol

TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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