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10269611001

Roche

Neuraminidase (Sialidase)

from Arthrobacter ureafaciens

Synonym(s):

PCR, taq

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1 UNIT
$382.00

$382.00

Available to ship onApril 29, 2025Details

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1 UNIT
$382.00

About This Item

Enzyme Commission number:
3.2.1.18 (BRENDA, IUBMB)
UNSPSC Code:
12352204

$382.00

Available to ship onApril 29, 2025Details

biological source

bacterial (Arthrobacter ureafaciens)

Quality Level

100

form

solution

specific activity

~25 units/mg protein

packaging

pkg of 1 U (100 μl)

manufacturer/tradename

Roche

optimum pH

5.0-5.5

storage temp.

2-8°C

General description

Neuraminidase hydrolyzes terminal N- or O-acylneuraminic acids which are α2,3-, α2,6-, or α2,8-linked (rate: α2,6 > α2,3 > α2,8) to oligosaccharides, polysaccharides, mucopolysaccharides, glycoproteins, and glycolipids. Noteworthy, for the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials.[1]

Specificity

Cleaves terminal sialic-acid residues that are α2,3-, α2,6-, or α2,8-linked to Gal, GlcNAc, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids, or glycoproteins. Relative rate of cleavage is α2,6 >α2,3 >α2,8, determined on bonds in tri- and tetrasaccharides.

Application

  • cell surface lectin array analysis.[2][3][4]
  • hemagglutination assays.[5]
  • cell adhesion assay.[6]
For the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials.
Neuraminidase has been used for the:
  • detection of the cell surface glycosylations in human anaplastic large cell lymphoma cells[2]
  • release of sialic acid from cells[7]
  • antibody-overlay lectin microarray[4]

Physical properties

This product is a mixture of isoenzymes (L, M1, M2 and S) with the following molecular weight values: ~ 52 kDa, 66 kDa and 88 kDa.

Physical form

Solution in 10 mM sodium phosphate, 0.1% Micr-O-Protect (w/v), 0.25 mg/ml bovine serum albumin, pH 7

Preparation Note

Working concentration: Enzyme/substrate ratio should be in the range of 0.04 U/25-80 μg.

Other Notes

For life science research only. Not for use in diagnostic procedures.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

wgk_germany

nwg

flash_point_f

does not flash

flash_point_c

does not flash

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Certificates of Analysis (COA)

Lot/Batch Number
64211820
53378321
29869420
19790821
19790820

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Charting the Proteoform Landscape of Serum Proteins in Individual Donors by High-Resolution Native Mass Spectrometry.
Cramer, et al.
Analytical Chemistry, 94, 12732-12741 (2022)
Chao Gao et al.
bioRxiv : the preprint server for biology (2020-08-09)
The spike (S) glycoprotein in the envelope of SARS-CoV-2 is densely glycosylated but the functions of its glycosylation are unknown. Here we demonstrate that S is recognized in a glycan-dependent manner by multiple innate immune receptors including the mannose receptor
Lam Raga Anggara Markely et al.
Biotechnology progress, 32(1), 235-241 (2015-11-21)
The serum half-life, biological activity, and solubility of many recombinant glycoproteins depend on their sialylation. Monitoring glycoprotein sialylation during cell culture manufacturing is, therefore, critical to ensure product efficacy and safety. Here a high-throughput method for semi-quantitative fingerprinting of glycoprotein
Cellular entry of the porcine epidemic diarrhea virus
Li W, et al.
Virus Research, 226, 117-127 (2016)
Tomonori Kaifu et al.
The Journal of experimental medicine, 218(12) (2021-11-25)
Dendritic cell immunoreceptor (DCIR) is a C-type lectin receptor with a carbohydrate recognition domain and an immunoreceptor tyrosine-based inhibitory motif. Previously, we showed that Dcir-/- mice spontaneously develop autoimmune enthesitis and sialadenitis, and also develop metabolic bone abnormalities. However, the
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Protocols

Neuraminidase (Sialidase) Protocol

Neuraminidase can be used to cleave sialic acids from proteins. In this protocol, the enzyme from Vibrio cholerae is used on fixed cells.

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