10269611001

Neuraminidase (Sialidase)

from Arthrobacter ureafaciens

• Synonym(s): PCR, taq
• Enzyme Commission number: 3.2.1.18 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: bacterial (Arthrobacter ureafaciens)
• Quality Level: 100
• form: solution
• specific activity: ~25 units/mg protein
• packaging: pkg of 1 U (100 μl)
• manufacturer/tradename: Roche
• optimum pH: 5.0-5.5
• storage temp.: 2-8°C

Description

General description

Neuraminidase hydrolyzes terminal N- or O-acylneuraminic acids which are α2,3-, α2,6-, or α2,8-linked (rate: α2,6 > α2,3 > α2,8) to oligosaccharides, polysaccharides, mucopolysaccharides, glycoproteins, and glycolipids. Noteworthy, for the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials.[1]

Specificity

Cleaves terminal sialic-acid residues that are α2,3-, α2,6-, or α2,8-linked to Gal, GlcNAc, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids, or glycoproteins. Relative rate of cleavage is α2,6 >α2,3 >α2,8, determined on bonds in tri- and tetrasaccharides.

Application

• cell surface lectin array analysis.[2][3][4]
• hemagglutination assays.[5]
• cell adhesion assay.[6]

For the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials.

Neuraminidase has been used for the:

• detection of the cell surface glycosylations in human anaplastic large cell lymphoma cells[2]
• release of sialic acid from cells[7]
• antibody-overlay lectin microarray[4]

Physical properties

This product is a mixture of isoenzymes (L, M1, M2 and S) with the following molecular weight values: ~ 52 kDa, 66 kDa and 88 kDa.

Physical form

Solution in 10 mM sodium phosphate, 0.1% Micr-O-Protect (w/v), 0.25 mg/ml bovine serum albumin, pH 7

Preparation Note

Working concentration: Enzyme/substrate ratio should be in the range of 0.04 U/25-80 μg.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
• Hazard Classifications: Skin Sens. 1
• wgk_germany: nwg
• flash_point_f: does not flash
• flash_point_c: does not flash