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10223581001

Roche

Poly(dA)

Poly-deoxy-adenylic acid

Synonym(s):

PCR

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About This Item

UNSPSC Code:
41105600

form

lyophilized

mol wt

330 Da

packaging

pkg of 5 U

manufacturer/tradename

Roche

absorbance ratio

A290/260 nm 0.03-0.12
A280/260 nm 0.28-0.39
A250/260 nm 0.84-0.94

storage temp.

−20°C (−15°C to −25°C)

General description

Poly(dA) is a polymer of polydeoxyadenylic acid and takes up a single stranded structure. It is susceptible to UV irradiation resulting in photoreaction due to the presence of adjacent deoxyadenylic acid units.[1] Poly(dA) along with β-glucan is used for the conjugation and delivery of DNA into cell lines.[2]

Application

Poly(dA) is suitable for use in:
  • DNA polymerase assay and as a buffer component for the dilution of protein DNA complex for electrophoretic mobility shift assay (EMSA) studies[3]
  • as ssDNA substrate for DNA helicase gene 40 product (G40P) NTP hydrolysis[4]
  • in Pyrococcus abyssi DNA polymerase assay[5]

Sequence

Chain Length Mean chain length lot 20-17: 250-500 bases.

Unit Definition

1 A260 unit corresponds to 40 μg ssDNA.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
11534322
11534321
14405323
14405320
14405322

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A novel gene carrier prepared from triple helical beta-glucan and polydeoxyadenylic acid
Liu Q, et al.
Journal of materials chemistry. B, 2(8), 933-944 (2014)
V Iyer et al.
The EMBO journal, 14(11), 2570-2579 (1995-06-01)
Many yeast promoters contain homopolymeric dA:dT sequences that affect nucleosome formation in vitro and are required for wild-type levels of transcription in vivo. Here, we show that poly(dA:dT) is a novel promoter element whose function depends on its intrinsic structure
The hyperthermophilic euryarchaeota Pyrococcus abyssi likely requires the two DNA polymerases D and B for DNA replication
Henneke G, et al.
Journal of molecular biology, 350(1), 53-64 (2005)
Human DNA polymerase lambda functionally and physically interacts with proliferating cell nuclear antigen in normal and translesion DNA synthesis
Maga G, et al.
The Journal of biological chemistry, 277(50), 48434-48440 (2002)
Bacillus subtilis bacteriophage SPP1 hexameric DNA helicase, G 40 P, interacts with forked DNA
Ayora S, et al.
Nucleic Acids Research, 30(11), 2280-2289 (2002)
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