10106399001

GTP

>90% (HPLC), crystals, pkg of 250 mg

• Synonym(s): Guanosine 5′-triphosphate sodium salt hydrate, 5′-GTP-Na₂
• Empirical Formula (Hill Notation): C₁₀H₁₆N₅O₁₄P₃ · xNa⁺ · yH₂O
• CAS Number: 36051-31-7
• Molecular Weight: 523.18 (anhydrous free acid basis)
• Beilstein/REAXYS Number: 4113439
• MDL number: MFCD00149227
• UNSPSC Code: 41106305
• PubChem Substance ID: 329748894

Properties

• description: Disodium salt
• Quality Level: 100
• assay: >90% (HPLC)
• form: crystals
• mol wt: M_r 523.2 (GTP); M_r 567.1 (GTP-Na₂)
• packaging: pkg of 250 mg
• manufacturer/tradename: Roche
• storage condition: avoid repeated freeze/thaw cycles
• SMILES string: O.O.[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1
• InChI: 1S/C10H16N5O14P3.2Na.2H2O/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;2*1H2/q;2*+1;;/p-2/t3-,5-,6-,9-;;;;/m1..../s1
• InChI key: HPLNHYUZFVUXFJ-ZVQJTLEUSA-L

Description

General description

GTP functions as a carrier of phosphates and pyrophosphates involved in channeling chemical energy into specific biosynthetic pathways. It also serves as an energy-rich precursor of mononucleotide units in the enzymatic biosynthesis of DNA and RNA. GTP is involved in the initiation of peptide synthesis during the binding of formylmethionyl-tRNA (F-met-tRNA) to the ribosome. It is also involved in polypeptide chain elongation. GTP is hydrolyzed by tubulin and its hydrolysis is accompanied by microtubule assembly.

GTP is supplied as the disodium salt in crystal form.

Application

GTP, disodium salt, is required as a coenzyme for protein biosynthesis in a cell-free system. It has been used in FtsZ polymerization assay and in the preparation of pipette solution for the electrophysiological recordings of the whole-cell membrane currents.

Quality

Purity: 76% GTP (enzymatic), >90 area% (HPLC), 9% Na⁺, 8% H2O

Preparation Note

Activator: EDTA prevents the formation of enzyme aggregates. Phosphate stimulates the NADH-dependent reaction.
Storage conditions (working solution): Aqueous solutions (pH 7.0) are stable at 2 to 8 °C or frozen for approx. one week, for reasons of hydrolysis pH should not be < 3.

If neutralized with water preparation can be stored frozen at -15 to -25 °C in small portions, one sample should thawed only once and should be used within the next one or two days (stored at 2 to 8 °C). Preparation can also be dissolved in a buffer pH 7-8 without neutralization (buffer concentration should be high enough that pH is not changed by addition of those substances); solution can be stored frozen.

Investigations on the storage temperature 2 to 8 °C compared to -15 to -25 °C showed no difference.

Storage and Stability

Store at 2 to 8 °C. (Store dry!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable