G5884

Guanosine 5′-triphosphate lithium salt

~95% (HPLC), powder

• Synonym(s): GTP lithium salt
• Empirical Formula (Hill Notation): C₁₀H₁₆N₅O₁₄P₃ · xLi⁺
• CAS Number: 85737-04-8
• Molecular Weight: 523.18 (free acid basis)
• MDL number: MFCD00070012
• UNSPSC Code: 41106305
• PubChem Substance ID: 24895236
• NACRES: NA.77

Properties

• assay: ~95% (HPLC)
• Quality Level: 200
• form: powder
• color: white
• solubility: H₂O: 100 mg/mL
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [Li+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP(O)(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1
• InChI: 1S/C10H16N5O14P3.Li/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);/q;+1/p-1/t3-,5-,6-,9-;/m1./s1
• InChI key: WDCLDKGIVWUKSE-GWTDSMLYSA-M

Description

Application

Guanosine 5′-triphosphate lithium salt has been used:

• In ribosome activity assay.[1]
• In tubulin isolation and polymerization.[2]
• In the determination of cell viability.[2]

Biochem/physiol Actions

GTP functions as a carrier of phosphates and pyrophosphates involved in channeling chemical energy into specific biosynthetic pathways. GTP activates the signal transducing G proteins which are involved in various cellular processes including proliferation, differentiation, and activation of several intracellular kinase cascades. Proliferation and apoptosis are regulated in part by the hydrolysis of GTP by small GTPases Ras and Rho. Another type of small GTPase, Rab, plays a role in the docking and fusion of vesicles and may also be involved in vesicle formation. In addition to its role in signal transduction, GTP also serves as an energy-rich precursor of mononucleotide units in the enzymatic biosynthesis of DNA and RNA.

Guanosine 5′-triphosphate serves as a substrate for guanosine 5′-triphosphate 3′ diphosphate diphosphatase, a hydrolyzing enzyme and guanosine triphosphate guanyltransferase.[3]

Features and Benefits

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

This compound is featured on the P2 Receptors: P2X Ion Channel Family page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Caution

Stability is much better than sodium salt.

Preparation Note

Prepared by enzymatic phosphorylation of 5′-GMP.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves