MABT332

Anti-Obscurin Antibody, clone 4A8

clone 4A8, from mouse

• Synonym(s): Obscurin, Obscurin-RhoGEF, Obscurin-myosin light chain kinase, Obscurin-MLCK
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4A8, monoclonal
• species reactivity: rat
• species reactivity (predicted by homology): human (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001092093
• UniProt accession no.: Q5VST9
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... OBSCN(84033)

Description

General description

Obscurin, also known as Obscurin-RhoGEF or Obscurin-myosin light chain kinase, is encoded by the gene name OBSCN. Obscurin is involved in myofibrillogenesis and the assembly of myosin into sarcomeric A bands in striated muscle. Obscurin isoform 3 plus ANK1 isoform Mu17/Ank1.5 may be molecular link between the sarcoplasmic reticulum and myofibrils.

Immunogen

GST-tagged recombinant protein corresponding to the N-terminus of human Obscurin.

Application

This Anti-Obscurin antibody is validated for use in WB, IH(P) for the detection of Obscurin.

Western Blotting Analysis: A representative lot detected Obscurin in extracts of adult rat quadriceps tissue lysate (Bowman, A.L., et al. (2007). FEBS Letters. 581:1549-1554).
Immunohistochemistry by fluorescent Analysis: A representative lot detected Isocitrate dehydrogenase in rat muscle tissue at resting stage (Bowman, A.L., et al. (2007). FEBS Letters. 581:1549-1554).

Quality

Evaluated by Immunohistochemistry in rat skeletal muscle tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Obscurin in rat skeletal muscle tissue.

Target description

800 kDa calculated

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable