MABN489
Anti-GABA(A)R, Alpha1 Protein Antibody, clone N95/35
clone N95/35, from mouse
Synonym(s):
Gamma-aminobutyric acid receptor subunit alpha-1, GABA(A) receptor subunit alpha-1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
N95/35, monoclonal
species reactivity
mouse, rat
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Gabra1(14394)
rat ... Gabra1(29705)
General description
The neurotransmitter GABA (gamma-aminobutyric acid) binds to specific transmembrane receptors in the plasma membrane of both pre- and postsynaptic neurons. Three general classes of GABA receptors are known: GABAA and GABAC (ionotropic) receptors and GABAB (metabotropic) receptors. The GABAA (gamma-Aminobutyric Acid type A) receptor is a multiprotein complex that acts as a ligand-gated chloride channel. These receptors are hetero-oligomers, for which 6 subunits are currently known (a, b, g, d, e and r). There are at least 6 different isoforms of the alpha subunit, and receptor subunit expression varies during development. Increased alpha1 subunit assembly in postsynaptic receptors of cerebellar inhibitory synapses is responsible for the fast inhibitory synaptic currents observed during postnatal development. Additionally, alpha1-containing GABAA receptors are important in regulating the reinforcing properties of alcohol, and expression of the alpha1 subunit increases upon chronic exposure to alcohol, making it a potential therapeutic target for alcohol dependency.
Specificity
This antibody recognizes the cytoplasmic domain of GABA(A)R, Alpha1 Protein. This antibody does not demonstrate cross-reactivity against GABA(A)R2 and GABA(A)R3 (Prof. J. Trimmer, University of California, Davis).
Immunogen
Epitope: Cytoplasmic domain
Recombinant protein corresponding to the cytoplasmic domain of mouse GABA(A)R, Alpha1 Protein.
Application
Immunohistochemistry Analysis: A representative lot detected GABA(A)R, Alpha1 Protein in rat hippocampus tissue (Prof. J. Trimmer, University of California, Davis).
Western Blot Analysis: A representative lot detected GABA(A)R, Alpha1 Protein in RBM membrane extracts, COS cell lysates transiently transfected with GABA(A)R, Alpha1 Protein, and in WT mice but not in GABA(A)R Alpha1 knockout mice.
Western Blot Analysis: A representative lot detected GABA(A)R, Alpha1 Protein in RBM membrane extracts, COS cell lysates transiently transfected with GABA(A)R, Alpha1 Protein, and in WT mice but not in GABA(A)R Alpha1 knockout mice.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
This Anti-GABA(A)R, Alpha1 Protein Antibody, clone N95/35 is validated for use in Western Blotting, IHC for the detection of GABA(A)R, Alpha1 Protein, clone N95/35.
Quality
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected GABA(A)R, Alpha1 Protein in 10 µg of mouse brain tissue lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected GABA(A)R, Alpha1 Protein in 10 µg of mouse brain tissue lysate.
Target description
~55 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse brain tissue lysate
Mouse brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Sarah Poliquin et al.
International journal of molecular sciences, 25(9) (2024-05-11)
A significant number of patients with genetic epilepsy do not obtain seizure freedom, despite developments in new antiseizure drugs, suggesting a need for novel therapeutic approaches. Many genetic epilepsies are associated with misfolded mutant proteins, including GABRG2(Q390X)-associated Dravet syndrome, which
Prabhuanand Selvaraj et al.
Cells, 10(12) (2021-12-25)
Modulation of the endocannabinoid system has emerged as an effective approach for the treatment of many neurodegenerative and neuropsychological diseases. However, the underlying mechanisms are still uncertain. Using a repetitive mild traumatic brain injury (mTBI) mouse model, we found that
Layane Hanna-El-Daher et al.
Neurobiology of disease, 79, 14-27 (2015-04-22)
Among cerebral creatine deficiency syndromes, guanidinoacetate methyltransferase (GAMT) deficiency can present the most severe symptoms, and is characterized by neurocognitive dysfunction due to creatine deficiency and accumulation of guanidinoacetate in the brain. So far, every patient was found with negligible
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