Glucose-6-phosphate isomerase (GPI) alternatively known as Autocrine motility factor (AMF), Neuroleukin (NLK), Phosphoglucose isomerase (PGI), Phosphohexose isomerase (PHI) or Sperm antigen 36 (SA-36) and encoded by the gene name GPI is major enzyme in glycolysis and gluconeogenesis. Glucose-6-phosphate isomerase rearranges glucose-6-phosphate (from the phosphorylation of glucose via hexokinases) into fructose-6-phosphate (F6P) as the second step in glycolysis. Interestingly however, Glucose-6-phosphate isomerase plays an entirely different sort of role outside of the cell than when it is inside being part of glycolysis. When secreted, Glucose-6-phosphate isomerase acts as an autocrine motility factor for various cancers and is critical for metastasis, while when secreted in nervous tissue it acts as a neurotrophic factor for sensory and spinal neurons and in lymphocytes it acts as a lymphokine upon T cell ligand binding and stimulates B cells to secrete immunoglobulin. Mutations in Glucose-6-phosphate isomerase are the second most frequent cause of inherited glycolytic-enzymopathy in humans. This autosomal recessive disorder is characterized by a non-spherocytic anemia of variable severity which can present with neuromuscular dysfunctions defined by muscle weakness and mental retardation.
Immunogen
GST-tagged recombinant protein corresponding to human Neuroleukin/GPI.
Application
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Neuroleukin/GPI in human thalamus and rat hindbrain tissues.
Research Category Neuroscience
Research Sub Category Signaling Neuroscience
This Anti-Neuroleukin/GPI Antibody, clone 10C2.1 is validated for use in western blotting & IHC (Paraffin) for the detection of Neuroleukin/GPI.
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 μg/mL of this antibody detected Neuroleukin/GPI in 10 μg of HeLa cell lysate.
Target description
~58 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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