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MABC580

Sigma-Aldrich

Anti-Clathrin Heavy Chain Antibody, clone 6F10.1

clone 6F10.1, from mouse

Synonym(s):

Clathrin heavy chain 1, Clathrin Heavy Chain, Clathrin heavy chain on chromosome 17, CLH-17

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

6F10.1, monoclonal

species reactivity

human, mouse, rat

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_004850

UniProt accession no.

Q00610

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CLTC(1213)

General description

Clathrin heavy chain 1 (UniProt Q00610; also known as Clathrin heavy chain on chromosome 17, Clathrin heavy polypeptide (Hc), Clathrin heavy polypeptide-like 2, CLH-17) is encoded by the CLTC (also known as CHC, CLH17, CLTCL2, KIAA0034) gene in human (Entrez Gene ID 1213). Clathrin is the major protein component of the polyhedral coat of coated pits and vesicles. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of various macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.

Immunogen

GST-tagged recombinant protein corresponding to human Clathrin Heavy Chain near the C-terminus.

Application

Anti-Clathrin Heavy Chain Antibody, clone 6F10.1 is an antibody against Clathrin Heavy Chain for use in Western Blotting, Flow Cytometry, Immunocytochemistry.
Flow Cytometry Analysis: 1.5 µg of this antibody from a representative lot detected Clathrin Heavy Chain in 1X10E6 Jurkat cells.
Immunocytochemistry Analysis: 25.0 µg/mL from a representative lot detected Clathrin Heavy Chain in HeLa, A431, HUVEC, and NIH/3T3 cells.

Quality

Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Clathrin Heavy Chain in 10 µg of PC12 cell lysate.

Target description

~190 kDa observed

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ping-Hung Chen et al.
Developmental cell, 40(3), 278-288 (2017-02-09)
Signaling receptors are internalized and regulated by clathrin-mediated endocytosis (CME). Two clathrin light chain isoforms, CLCa and CLCb, are integral components of the endocytic machinery whose differential functions remain unknown. We report that CLCb is specifically upregulated in non-small-cell lung
Hui Li et al.
Journal of extracellular vesicles, 9(1), 1812261-1812261 (2020-09-19)
Pregnancy is a unique situation, in which placenta-derived small extracellular vesicles (sEVs) may communicate with maternal and foetal tissues. While relevant to homoeostatic and pathological functions, the mechanisms underlying sEV entry and cargo handling in target cells remain largely unknown.

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