MAB1789
Anti-Migration Inhibitory Factor Related Proteins 8 and 14 Antibody, clone AHN-17
clone AHN-17, Chemicon®, from mouse
Synonym(s):
MRP-8 & 9, Myeloid-Related Protein-8 & 9, Calgranulin A & B, S100 Calcium-binding Protein A8 & A9, Leukocyte L1 cplx Light & Heavy Chain, Calprotectin
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100 μG
$414.00
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
AHN-17, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... S100A8(6279)
General description
Protein S100-A8/9 (UniProt: P05109/ P06702; also known as MRP-8/MRP-14, Leukocyte L1 complex light chain/ Leukocyte L1 complex heavy chain, p8/p14, S100 calcium-binding protein A8/A9) are encoded by the S100A8/S100A9 (also known as MRP8/MRP14) genes (Gene ID: 6279/6280) in human. MRP-8 and MRP-14 are calcium- and zinc-binding protein that play a prominent role in the regulation of inflammatory processes and immune response. They are known to induce neutrophil chemotaxis and adhesion. They are predominantly found as calprotectin and perform a variety of intra- and extracellular functions. Their intracellular functions include facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes, and activation of the neutrophilic NADPH-oxidase. They are known to activate NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane. MRP-8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. Their extracellular functions include proinflammatory, antimicrobial, oxidant-scavenging and induction of apoptosis. MRP-14 is reported to induce neutrophil chemotaxis and adhesion and can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 pathways and can induce degranulation of neutrophils by a MAPK-dependent mechanism. MRP-8 and MRP-14 also acts as danger associated molecular pattern (DAMP) molecules and stimulate innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation end (AGER) products. They also play an impotent role as oxidant scavengers and have a protective role in preventing exaggerated tissue damage by scavenging oxidants. (Ref.: Atallah, M., et al. (2012). PloS One. 7(2); e29333; Nakatani, Y., et al. (2005). Mediators Inflamm. 2005(5); 280-292; Ryckman, C., et al. (2003). J. Immunol. 170(6); 3233-3242).
Specificity
Migration Inhibitory Factor Releasing Proteins -8 and -14
Immunogen
Neutrophils
Application
Detect Migration Inhibitory Factor Related Proteins 8 & 14 using this Anti-Migration Inhibitory Factor Related Proteins 8 & 14 Antibody, clone AHN-17 validated for use in WB & IC.
Immunocytochemistry on ethanol fixed cells
Immunoblotting on un-reduced gels
Optimal dilutions must be determined by the end user.
Immunoblotting on un-reduced gels
Optimal dilutions must be determined by the end user.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
Cytokines & Cytokine Receptors
Linkage
Replaces: CBL269
Physical form
Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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