HTS016M
ChemiSCREEN C3aR Membrane Preparation
Human C3aR GPCR membrane preparation for Radioligand binding Assays.
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32161000
NACRES:
NA.84
$879.00
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biological source
human
Quality Level
recombinant
expressed in Chem-2 cells
manufacturer/tradename
ChemiScreen
Chemicon®
concentration
0.5 mg/mL
technique(s)
radioligand binding assay (RLBA): suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
General description
C3a, along with C4a and C5a, is a 77 amino acid anaphylotoxin generated by proteolytic cleavage during activation of the complement pathway (Ember, 1997). The anaphylotoxins strongly promote inflammation by recruiting leukocytes, particularly basophils, eosinophils, neutrophils and monocyte (Martin, 1997). The proinflammatory properties of C3a are mediated by interaction between the peptide and a 7-TM G-protein coupled receptor, C3aR (Crass, 1996). Genetic and pharmacological inhibition of C3a/C3aR interaction indicates an important role for C3aR in allergic asthsma Humbles, 2000; Drouin, 2002). C3a/C3aR also enhances the effect of SDF-1 in promoting retention of haematopoietic stem/progenitor cells within the bone marrow (Rataiczak, 2004). Chemicon′s C3aR membrane preps are an ideal tool for screening for compounds that disrupt binding of C3a to C3aR. The membranes have been shown to bind to 125I-labeled C3a with a Kd of 0.06 nM.
Full-length human C3aR cDNA
Application
Human C3aR GPCR membrane preparation for Radioligand binding Assays.
Radioligand binding assay
Biochem/physiol Actions
GPCR Class: A
Protein Target: C3aR
Target Sub-Family: Anaphylotoxin
Quality
Bmax for [125I]C3a binding: 1.9 pmol/mg protein
Kd for [125I] C3a binding: ~ 0.06 nM
Kd for [125I] C3a binding: ~ 0.06 nM
Physical form
Membranes were adjusted to 0.5mg protein/mL in 50 mM Tris-HCL, pH 7.4, 10% glycerol and 1% BSA, rapidly frozen, and stored at -80°C. One vial contains 500 units, where a unit is the amount of membrane that will yield greater than 1000 cpm specific binding of 125I-labeled C3a (0.04 nM input concentration).
Storage and Stability
Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
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