G protein-regulated inducer of neurite outgrowth 1 (UniProt Q7Z2K8; also known as GRIN1) is encoded by the GPRIN1 (also known as KIAA1893) gene (Gene ID 114787) in human. Originally identified as a binding protein for guanosine 5′-3-O-(thio)triphosphate-bound G(z)alphaz, G protein-regulated inducer of neurite outgrowth 1 (GPRIN1) is a signaling molecule that functions as a downstream target of G(o)alpha. When co-expressed with constitutively active form of G(o)alpha, GPRIN1 is demonstrated to stimulate neurite extensions in Neuro2a cells. Gprin1 expression in mice is limited to the nervous system at all developmental stages, occurring within the entire embryonic mantle zones and becoming restricted to sets of nuclei at postnatal to adult stages. Gprin1 immunoreactivity is found colocalized with G(o)alpha at neuronal dendrites and axons, while being undetected in glial cells. Studies conducted in rat reveals that 7 nicotinic receptor ( 7) significantly reduces axon growth in hippocampal neurons by coupling to Gprin1, which targets it to the growth cone. RNAi-mediated Gprin1 knockdown abolishes the localization and calcium signaling of 7 at the growth cone. The 7/Gprin1 interaction appears intimately linked to a G(o)alpha, growth-associated protein 43, and CDC42 cytoskeletal regulatory pathway within the developing axon.
Specificity
This rabbit polyclonal antibody targets an internal sequence present in both spliced isoforms of human GPRIN1 protein reported by UniProt (Q7Z2K8).
Immunogen
KLH-conjugated linear peptide corresponding to an internal sequence of human GPRIN1.
Application
Anti-GPRIN1 Antibody, Cat. No. ABN1446, is a highly specific rabbit polyclonal antibody that targets GPRIN1 and has been tested in Immunohistochemistry and Western Blotting.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GPRIN1 in human prostate cancer, cerebral cortex and pancreas tissue sections.
Quality
Evaluated by Western Blotting in HT-29 cell lysate.
Western Blotting Analysis: A 1:20,000 dilution of this antibody detected GPRIN1 in 10 µg of HT-29 cell lysate.
Target description
~105 kDa observed. 102.4 kDa (isoform 1) and 80.95 kDa (isoform 2) calculated. Uncharacterized bands may be observed in some lysate(s).
Other Notes
Concentration: Please refer to lot specific datasheet.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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