374817

Hemocyanin, Keyhole Limpet, Megathura crenulata, Glycerol

Hemocyanin, Keyhole Limpet, Megathura crenulata, Glycerol, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

• Synonym(s): Hemocyanin, Keyhole Limpet, Megathura crenulata, Glycerol, KLH
• CAS Number: 9013-72-3
• MDL number: MFCD00131280
• UNSPSC Code: 12352202
• NACRES: NA.25

Properties

• Quality Level: 100
• assay: >90% (size exclusion chromatography)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: clear blue-gray
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Carrier protein that has many available primary amines to facilitate protein conjugation. Used to immunize animals to elicit antibodies to small molecules (haptens) by covalent conjugation. Protein: ≥10 mg/ml. Sold on the basis of protein content.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 10 mM MgSO₄, 5 mM BES, 50% glycerol, 0.9% sodium chloride, pH 6.5.

Preparation Note

Further dilute with PBS or physiological saline. Material can be dialyzed to remove the glycerol or used as is for conjugation with glutaraldehyde, carbodiimide, or mixed anhydrides.KLH is an extremely large protein complex that has a tendency to aggregate and precipitate from solutions. Use a buffer when diluting KLH and maintain the magnesium concentration at ≥10 mM. Store KLH solutions at 4°C. DO NOT freeze, vortex or agitate to dissolve KLH. Freezing or agitating KLH will cause protein aggregation and precipitation.

Other Notes

Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol.63, 37.
Shuler, K.R., et al. 1992. J. Immunol. Methods156, 137.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2