324414

Dynamin Inhibitor V, 34-2

The Dynamin Inhibitor V, 34-2 controls the biological activity of Dynamin. This small molecule/inhibitor is primarily used for Membrane applications.

• Synonym(s): Dynamin Inhibitor V, 34-2, 2-Cyano-N-octyl-3-(1-(3-dimethylaminopropyl)-1H-indol-3-yl)-acrylamide
• Empirical Formula (Hill Notation): C₂₅H₃₆N₄O
• CAS Number: 1128165-88-7
• Molecular Weight: 408.58
• MDL number: MFCD19690957
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• assay: ≥99% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: yellow
• solubility: DMSO: 100 mg/mL, clear, yellow
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: O=C(NCCCCCCCC)/C(C#N)=C/C1=CN(CCCN(C)C)C2=C1C=CC=C2

Description

General description

A cell-permeable, allosteric Dynamin inhibitor (IC₅₀ = 6.9 µM and 14 µM for full length Dynamin I and Dynamin II GTPase activity, respectively, in vitro). 10 µM of this compound results in cytokinesis failure at the abscission stage in HeLa cultures, with 82% of cells that enter into mitosis becoming multinucleated, and with 97% resulting in cell death. However, it displays a much reduced effect in NIH3T3 cells at the same concentration. It elicits a dose-dependent reduction in cell growth against SMA-560, SJ-G2, SW480, MCF-7 and A2780 cultures, as being the most sensitive among a panel of other cancer cell lines (GI₅₀ = 4.0–37 µM). It was further shown to be 6-fold more active (IC₅₀ = 5.0 µM) than dynamin mediated receptor mediated endocytosis (RME) in U2OS cells, and 15-fold more active (IC₅₀ = 1.3 µM) than Dynasore (Cat. No. 324410) in another Dynamin I GTPase activity assay.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Chircop, M. et al. 2011. Mol. Cancer Ther.10, 1553.
Hill, T.A., et al. 2009. J. Med. Chem.52, 3762.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable