1.10218

Gram′s decolorizing solution

for the Gram staining method

• UNSPSC Code: 41116130
• NACRES: NA.74

Properties

• Quality Level: 400
• form: liquid
• autoignition temp.: >425 °C
• IVD: for in vitro diagnostic use
• transition temp: flash point -10 °C
• density: 0.79 g/cm³ at 20 °C
• application(s): clinical testing; diagnostic assay manufacturing; hematology; histology
• storage temp.: 15-25°C

Description

General description

Gram′s decolorizing solution - for the Gram staining method, is a ready-to-use solution used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram′s crystal violet solution (Product number 1.09218) - an aniline dye - in the first step. After the treatment with iodine solution (Lugol′s solution stabilized with PVP, Product number 1.00567), a dye-iodine complex will form. During the decolorizing step (Gram′s decolorizing solution, Product number 1.10218), this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue-violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram′s safranin solution (Gram′s safranine solution (Product number 1.09217) and appear pink to red.
A bottle with 500 ml Gram′s decolorizing solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Product number 1.11885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU).

Analysis Note

Suitability for microscopy: passes test
Staphylococcus aureus: dark blue-violet
E.coli: pink to red

Safety Information

• pictograms: GHS02,GHS07
• signalword: Danger
• hcodes: H225 - H319 - H336
• pcodes: P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
• target_organs: Central nervous system
• Storage Class: 3 - Flammable liquids
• wgk_germany: WGK 1
• flash_point_f: 14.0 °F
• flash_point_c: -10 °C