1.01350

Leishman′s eosine methylene blue

for microscopy

• UNSPSC Code: 41116130
• NACRES: NA.21

Properties

• Quality Level: 400
• form: solid
• IVD: for in vitro diagnostic use
• pH: 5-6 (0.01% in methanol: water (1:1))
• bulk density: 700 kg/m³
• εmax: 650-850 at 522-525 nm in methanol at 0.0005%; 800-1100 at 623-627 nm in methanol at 0.0005%
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C

Description

General description

The dry mixture of the Leishman′s eosin methylene blue dye for microscopy, is used for the self-preparation of the Leishman′s staining solution. The staining method according to Leishman is one of the standard techniques in hematological diagnostic procedures. Like Wright′s eosin methylene blue and May-Grünwald′s eosin methylene blue, it is used for staining of blood and bone marrow smears and clinical cytological specimens. The dye is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Identity (UV/VIS-Spectrum): passes test
pH-value (0,01 %; methanol 50 %): 5 - 6
Absorption maximum λ 1 (Methanol): 623 - 627 nm
Absorption maximum λ 2 (Methanol): 522 - 525 nm
Spec. Absorptivity A 1%/1cm
- λ1_max; 0.0005 %; methanol; calculated on dried substance:
800 - 1100
- λ2_max; 0.0005 %; methanol; calculated on dried substance: 650 - 850
TLC-Test: passes test
Loss on drying (105 °C): ≤ 8 %
Suitability for microscopy: passes test

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H302 - H317 - H319
• pcodes: P261 - P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3