07-131
Anti-Sirt1(Sir2) Antibody
Upstate®, from rabbit
Synonym(s):
SIR2-like protein 2, silencing information regulator 2-like, silent information regulator 2, sir2-related protein type 2, sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae), sirtuin (silent mating type information regulation
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All Photos(5)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... SIRT1(23411)
mouse ... Sirt1(93759)
General description
Sirtuins are a family of closely related enzymes named after the yeast gene Sir2 (silent mating type information regulation 2). Members of the Sirtuin family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organism′s response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". Sirtuins have been implicated in the regulation of aging, transcription, apoptosis and stress resistance. Sirt1 (also known as Sirtuin 1, Sir2L1, and Sir2a), the mammalian homolog of yeast Sir2, has been shown to deacetylate and thereby deactivate the p53 protein to regulate cellular processes.
Specificity
This antibody recognizes Sirt1.
Immunogen
Epitope: a.a. 1-131
GST-tagged fusion protein corresponding to amino acids 1-131 of mouse Sir2α.
Application
Immunoprecipitation:
5 µL of a previous lot of this antibody has been shown to immunoprecipitate Sirt1 from 1 mg of a 3T3/A31 RIPA cell extract.
Immunocytochemistry:
This antibody has been reported to detect Sirt1 in 3T3 cells fixed with paraformaldehyde.
Chromatin Immunoprecipitation:
Published by Tiberi et al., (2012)
5 µL of a previous lot of this antibody has been shown to immunoprecipitate Sirt1 from 1 mg of a 3T3/A31 RIPA cell extract.
Immunocytochemistry:
This antibody has been reported to detect Sirt1 in 3T3 cells fixed with paraformaldehyde.
Chromatin Immunoprecipitation:
Published by Tiberi et al., (2012)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-Sirt1(Sir2) Antibody (Rabbit Polyclonal Antibody) is published (more than 30 citations) and validated in ICC, IP, WB to detect Sirt1(Sir2) also known as Silencing information regulator 2-like, sirtuin 2.
Quality
Routinely evaluated by western blot on 3T3 nuclear extract.
Western Blot Analysis:
A 1:1000-1:5000 dilution of this lot detected Sirt1 in a 3T3 nuclear extract.
Western Blot Analysis:
A 1:1000-1:5000 dilution of this lot detected Sirt1 in a 3T3 nuclear extract.
Target description
110 kDa; Uncharacterized bands 70 and 50 observed in some Lysates.
Linkage
Replaces: 05-707
Physical form
Immunoaffinity Chromatography
Purified rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide and 30% gylcerol
Storage and Stability
1 year at -20°C from date of shipment.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
Jurkat cell lysate, human colon carcinoma or human lung squamous carcinoma.
Jurkat cell lysate, human colon carcinoma or human lung squamous carcinoma.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Enterocyte-specific inactivation of SIRT1 reduces tumor load in the APC(+/min) mouse model.
Leko, V; Park, GJ; Lao, U; Simon, JA; Bedalov, A
Testing null
A deacetylase-deficient SIRT1 variant opposes full-length SIRT1 in regulating tumor suppressor p53 and governs expression of cancer-related genes.
Shah, ZH; Ahmed, SU; Ford, JR; Allison, SJ; Knight, JR; Milner, J
Molecular and cellular biology null
Short-term adenosine monophosphate-activated protein kinase activator 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranoside treatment increases the sirtuin 1 protein expression in skeletal muscle.
Suwa M, Nakano H, Radak Z, Kumagai S
Metabolism, Clinical and Experimental null
Activation of stress response gene SIRT1 by BCR-ABL promotes leukemogenesis.
Yuan, H; Wang, Z; Li, L; Zhang, H; Modi, H; Horne, D; Bhatia, R; Chen, W
Blood null
Human SIRT1 associates with mitotic chromatin and contributes to chromosomal condensation.
Samuel T Fatoba,Andrei L Okorokov
Cell Cycle null
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