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06-1125

Sigma-Aldrich

Anti-Ipaf (NLRC4) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

NLR family, CARD domain containing 4, NOD-like receptor C4, caspase recruitment domain family, member 12, ICE-protease activating factor, nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4, NLR family CARD domain-

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

NP_067032

UniProt accession no.

Q9NPP4

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GNL3(26354)

General description

Ipaf, also known as NLRC4, is a human CED-4 homologue and is thought to be an activator of pro-caspase-1 and might also be associated with the activation of caspase-1 as a response to pro-inflammatory and apoptotic stimulus. It has been shown to be induced by p53 and contributes to p53-dependent apoptosis. Ipaf is thought to play a role in the activation of the innate immune system.

Specificity

This antibody recognizes Ipaf at the LRR4 domain.

Immunogen

Epitope: LRR4 domain
KLH-conjugated linear peptide corresponding to human Ipaf (NLRC4) at the LRR4 domain.

application

Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-Ipaf (NLRC4) Antibody is validated for use in IC, WB for the detection of Ipaf (NLRC4).
Western Blot Analysis: 1 ug/ml of this antibody detected Ipaf in 10 ug of human lung tissue lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Ipaf in NIH/3T3 cells.

Quality

Evaluated by Western Blot in human lung tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Ipaf in 10 µg of human lung tissue lysate.

Target description

~112 kDa observed.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human lung tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Congfei Xu et al.
Nature communications, 9(1), 4092-4092 (2018-10-07)
The NLRP3 inflammasome is a well-studied target for the treatment of multiple inflammatory diseases, but how to promote the current therapeutics remains a large challenge. CRISPR/Cas9, as a gene editing tool, allows for direct ablation of NLRP3 at the genomic
An activating NLRC4 inflammasome mutation causes autoinflammation with recurrent macrophage activation syndrome.
Canna, SW; de Jesus, AA; Gouni, S; Brooks, SR; Marrero, B; Liu, Y; DiMattia, MA; Zaal et al.
Nature Genetics null
Ho Yin Pekkle Lam et al.
Parasitology international, 77, 102119-102119 (2020-04-14)
Angiostrongylus cantonensis is a metastrongyloid nematode that causes eosinophilic meningoencephalitis in humans. A high infestation of A. cantonensis can cause permanent brain damage or even death. The inflammasome is an oligomeric molecular platform that can detect microbial pathogens and activate
Changhoon Oh et al.
Cell reports, 41(8), 111688-111688 (2022-11-24)
In neutrophils, caspase-11 cleaves gasdermin D (GSDMD), causing pyroptosis to clear cytosol-invasive bacteria. In contrast, caspase-1 also cleaves GSDMD but seems to not cause pyroptosis. Here, we show that this pyroptosis-resistant caspase-1 activation is specifically programmed by the site of
Xiangyang Xiong et al.
Frontiers in neuroscience, 16, 816752-816752 (2022-03-22)
Sleep deprivation (SD) induces systemic inflammation that promotes neuronal pyroptosis. The purpose of this study was to investigate the effect of an antioxidant modafinil on neuronal pyroptosis and cognitive decline following SD. Using a mouse model of SD, we found
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