330021S
Avanti
WR304 mAb
Avanti Research™ - A Croda Brand 330021S
Synonym(s):
WR304 mAb, WR304 Mouse Monoclonal Antibody (IgM), Anti-(PIP/PIP2), 1X TBS (Tris Buffered Saline) with 1mM Na2 EDTA
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
antibody form
purified immunoglobulin
antibody product type
primary antibodies
assay
>95%
packaging
pkg of 1 × 0.1 mL (polypropylene tube with screw cap (330021S-100ug))
manufacturer/tradename
Avanti Research™ - A Croda Brand 330021S
concentration
1 mg/mL (330021S-100ug)
shipped in
dry ice
storage temp.
−70°C
General description
WR304 is an antibody that binds specifically to the lipids PIP and PIP2. WR304 also neutralizes infectious HIV-1 virus and can be used to probe the presence of PIP and PIP2 in membranes in a variety of applications including inositol phosphate-dependent cell signaling pathways. This antibody was developed by the Walter Reed Army Institute of Research (WRAIR) using a targeted adjuvant protocol with liposomes containing Avanti′s Lipid A.
Formulation: WR304 is provided as a 0.2μm sterile-filtered solution in Tris buffered saline (TBS). WR304 binding to porcine brain PI(4)P is inhibited by high concentration of Ca2+ (10 mM)iv and addition of 1 mM EDTA stimulates this binding activity. PBS and other phosphate-containing buffers should not be used as they inhibit binding to the phospholipid head groupiii.
Formulation: WR304 is provided as a 0.2μm sterile-filtered solution in Tris buffered saline (TBS). WR304 binding to porcine brain PI(4)P is inhibited by high concentration of Ca2+ (10 mM)iv and addition of 1 mM EDTA stimulates this binding activity. PBS and other phosphate-containing buffers should not be used as they inhibit binding to the phospholipid head groupiii.
Specificity
WR304 recognizes PIP and PIP2, and neutralizes HIV-1 virus.
Immunogen
Antigens: Brain PI(4)P
Application
ELISA, IHC, Dot Blot, Flow cytometry and HIV-1 infectivity.
The WR304 antibody has been used for the quantitation of PIP/PIP2 in direct ELISA and Dot Blot analysis. The WR304 antibody specifically binds to PIP/PIP2 and inhibits the infectivity of HIV-1 to peripheral blood mononuclear cells (PBMC).
The WR304 antibody has been used for the quantitation of PIP/PIP2 in direct ELISA and Dot Blot analysis. The WR304 antibody specifically binds to PIP/PIP2 and inhibits the infectivity of HIV-1 to peripheral blood mononuclear cells (PBMC).
Packaging
0.5 mL Polypropylene Tube with Screw Cap (330021S-100ug)
Storage and Stability
Store undiluted at either -20°C or -80°C.
Other Notes
Hazardous/Non-hazardous Components: This product contains no substances that, at their given concentration, are known to be hazardous to health. Therefore, there is no MSDS for this product.
Ig Class: Mouse IgM (kappa).
Antibody Source: Monoclonal antibody from BALB/c-derived hybridoma WR304.
Production: In vitro cell culture. Purification: Ultra filtration through 100 KDa cut-off filters.
Purification: Ultra filtration through 100 KDa cut-off filters.
Antibody Source: Monoclonal antibody from BALB/c-derived hybridoma WR304.
Production: In vitro cell culture. Purification: Ultra filtration through 100 KDa cut-off filters.
Purification: Ultra filtration through 100 KDa cut-off filters.
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC
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Storage Class
10 - Combustible liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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N M Wassef et al.
Molecular immunology, 21(10), 863-868 (1984-10-01)
Monoclonal antibodies against phosphatidylinositol phosphate were produced after injecting a mouse with liposomes containing dimyristoylphosphatidylcholine, cholesterol, phosphatidylinositol phosphate and lipid A. The antibodies raised were IgM (kappa) and their activities were assayed by complement-dependent damage to liposomes lacking lipid A
Zoltan Beck et al.
Biochemical and biophysical research communications, 354(3), 747-751 (2007-01-30)
The binding characteristics of two monoclonal antibodies (mAb) to phosphatidylinositol-4-phosphate (PIP) were examined: a murine IgM mAb to PIP; and a human IgG mAb (4E10) that binds both to HIV-1 envelope protein and also to neutral and anionic phospholipids, including
Gary R Matyas et al.
Biochimica et biophysica acta, 1788(3), 660-665 (2008-12-23)
Two human mAbs (2F5 and 4E10), originally derived from HIV-1-infected patients, are important, but rare, mAbs that exhibit broad cross-clade neutralizing activities against HIV-1. In addition to peptide sequences on the gp41 envelope protein, both antibodies reportedly also bound specifically
Bruce K Brown et al.
Journal of virology, 81(4), 2087-2091 (2006-12-08)
Both a murine monoclonal antibody to phosphatidylinositol phosphate (PIP) and a human monoclonal antibody (4E10) that is known to have broadly neutralizing capabilities against primary isolates of human immunodeficiency virus type 1 (HIV-1) bound to PIP, as determined by enzyme-linked
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