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227935

Sigma-Aldrich

Azure B

prepared by direct synthesis

Synonym(s):

N,N,N′-Trimethylthionin, Azure I, Methyleneazure

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100 G
$2,770.00

About This Item

Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
531-55-5
Molecular Weight:
305.83
Colour Index Number:
52010
Beilstein/REAXYS Number:
3922630
EC Number:
208-511-2
MDL number:
MFCD00011935
UNSPSC Code:
12171500
PubChem Substance ID:
24853664
NACRES:
NA.47

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assay

>85.0% (HPLC)

form

powder

mp

205-210 °C (dec.) (lit.)

solubility

water: 1 mg/mL, blue to very deep blue

λmax

648 nm

ε (extinction coefficient)

≥13000 at 241-247 nm
≥36000 at 287-293 nm
≥67000 at 638-644 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

C[N+](C)=C(C=C1)C=C(C1=N2)SC3=C2C=CC(NC)=C3.[Cl-]

InChI

1S/C15H15N3S.ClH/c1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;/h4-9H,1-3H3;1H

InChI key

DNDJEIWCTMMZBX-UHFFFAOYSA-N

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Application

Azure B has been used in M′Fadyean stain to visualize Bacillus anthracis capsule.[1]

Biochem/physiol Actions

Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for staining of blood films.[2] It is also used for the localization of nucleic acids and lignin.[3]

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS3419
SHBS2077
SHBS1526
SHBS1846
MKCN7451

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U.S. Forest Service research note FPL (1964)
A simple, reliable M'Fadyean stain for visualizing the Bacillus anthracis capsule
Owen MP
Journal of Microbiological Methods, 92, 264-269 (2013)
Barbara J. Bain, Imelda Bates, Mike A Laffan
Dacie and Lewis Practical Haematology (2016)
Luaine Bandounas et al.
New biotechnology, 30(2), 196-204 (2012-05-09)
In this study we have investigated the molecular background of the previously reported dye decolourization potential of Bacillus sp. LD003. Strain LD003 was previously isolated on Kraft lignin and was able to decolourize various lignin model dyes. Specifically Azure B
Susana Silva Martínez et al.
Ultrasonics sonochemistry, 19(1), 174-178 (2011-06-15)
The degradation of azure B dye (C15H16ClN3S; AB) has been studied by Fenton, sonolysis and sono-electroFenton processes employing ultrasound at 23 kHz and the electrogeneration of H2O2 at the reticulated vitreous carbon electrode. It was found that the dye degradation
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