200182

Nitrazine Yellow

indicator grade, Dye content 85 %

• Synonym(s): 2-(2,4-Dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt, 2-(2,4-Dinitrophenylazo)naphthol-3,6-disulfonic acid disodium salt, Nitrazol Yellow
• Empirical Formula (Hill Notation): C₁₆H₈N₄Na₂O₁₁S₂
• CAS Number: 5423-07-4
• Molecular Weight: 542.36
• Colour Index Number: 14890
• EC Number: 226-549-8
• MDL number: MFCD00003938
• UNSPSC Code: 12171500
• PubChem Substance ID: 24851961
• NACRES: NA.47

Properties

• grade: indicator grade
• assay: >90.0% (HPLC)
• form: powder
• composition: Dye content, 85%
• solubility: H₂O: 1 mg/mL
• λmax: 586 nm
• ε (extinction coefficient): ≥35000 at 587-591 nm in 0.1 M NaOH at 0.01 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].Oc1c(\N=N\c2ccc(cc2[N+]([O-])=O)[N+]([O-])=O)c(cc3cc(ccc13)S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C16H10N4O11S2.2Na/c21-16-11-3-2-10(32(26,27)28)5-8(11)6-14(33(29,30)31)15(16)18-17-12-4-1-9(19(22)23)7-13(12)20(24)25;;/h1-7,21H,(H,26,27,28)(H,29,30,31);;/q;2*+1/p-2/b18-17+;;
• InChI key: XBLIFEQTVVSTIM-QIKYXUGXSA-L

Description

Application

Nitrazine Yellow has been used in dye-protein binding assay.

Biochem/physiol Actions

Nitrazine Yellow ( 2-(2,4-dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt) is a pH-sensitive azo dye. It changes color from yellow to blue in pH range 6.0–7.0 and is widely used in microscopy. Throat swab immersed in nitrazine yellow is used to identify pH variations in the vagina before and after amniotomy.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• ppe: Eyeshields, Gloves, type N95 (US)