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Sigma-Aldrich

Nigrosin

certified by the Biological Stain Commission

Synonym(s):

Acid black 2, Nigrosin water soluble

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About This Item

CAS Number:
8005-03-6
Colour Index Number:
50420
MDL number:
MFCD00044681
UNSPSC Code:
12171500
NACRES:
NA.47

grade

certified by the Biological Stain Commission

form

solid

solubility

H2O: 0.1%
H2O: soluble

λmax

570 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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Suitability

Certified for use in Dorner′s spore stain. Used as a negative stain in place of India ink for spirochetes, bacteria, protozoa and fungi.
Used as a negative stain in place of India ink for spirochetes, bacteria, protozoa and fungi.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lina Hacker et al.
Journal of biomedical optics, 25(9) (2020-09-06)
Photoacoustic imaging (PAI) enables the detection of blood hemoglobin (HB) concentration and oxygenation (sO2) with high contrast and resolution. Despite the heavy use of photoacoustically determined total hemoglobin (THb) and oxygenation (sO2) biomarkers in PAI research, their relationship with underlying
Chris Campbell et al.
Biomedical optics express, 11(10), 5800-5813 (2020-11-06)
Hemozoin (Hz) is a crystal by-product of hemoglobin consumption by malaria parasites. There are currently no in vivo deep tissue sensing methods that can quantify Hz presence noninvasively, which would be advantageous for malaria research and treatment. In this work
Marcel Gehrung et al.
Journal of biomedical optics, 24(12), 1-11 (2019-10-19)
Photoacoustic tomography (PAT) is intrinsically sensitive to blood oxygen saturation (sO2) in vivo. However, making accurate sO2 measurements without knowledge of tissue- and instrumentation-related correction factors is extremely challenging. We have developed a low-cost flow phantom to facilitate validation of
Jesse Schallek et al.
Investigative ophthalmology & visual science, 52(3), 1325-1335 (2010-11-06)
To examine the extent to which neurovascular coupling contributes to stimulus-evoked intrinsic signals in the retina. The retinas of five adult cats were examined in vivo. Animals were anesthetized and paralyzed for imaging stability. The retinas were imaged through a
G E Freneau et al.
Animal reproduction science, 118(2-4), 176-181 (2009-09-30)
The objectives of this study were to compare two different methods of evaluating bull sperm morphology, bright-field (BF) microscopy of eosin-nigrosin (EN) stained dry-mount semen smears and differential interference phase contrast (DIC) microscopy of wet-mount semen 'fixed' in isotonic formal
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