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163783

Sigma-Aldrich

Tricine

≥98%

Synonym(s):

N-[Tris(hydroxymethyl)methyl]glycine

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About This Item

Linear Formula:
(HOCH2)3CNHCH2CO2H
CAS Number:
5704-04-1
Molecular Weight:
179.17
Beilstein/REAXYS Number:
1937804
EC Number:
227-193-6
MDL number:
MFCD00004277
UNSPSC Code:
12352200
PubChem Substance ID:
329751205
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assay

≥98%

useful pH range

7.4-8.8

pKa (25 °C)

8.1

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

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General description

Tricine is one of the Good′s buffers that derives its name from tris and glycine. It has a pKa value of 7.8 and commonly used in animal tissue culture.[1] It is a very useful buffer for the separation of small proteins in the range of 1 to 100 kDa.[2][3]

Application

Buffer component for separation of low molecular weight peptides.
Tricine is a buffer useful in the physiological pH range 7.4-8.8 and pKa=8.15 at 20°C.[4][5] It can also be used in many electrophoretic techniques for the analysis of proteins.[6][7]

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Certificates of Analysis (COA)

Lot/Batch Number
09611DW
00513CX
08014BX
07609LT
01512LV

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Detection of prion protein using a capillary electrophoresis-based competitive immunoassay with laser-induced fluorescence detection and cyclodextrin-aided separation
Yang W, et al.
Electrophoresis, 26(9), 1751-1759 (2005)
The use of tricine buffer in animal tissue cultures
Gardner R S
The Journal of cell biology, 42(1), 320-320 (1969)
Bioluminescent determination of 0.1 picomole amounts of guanine nucleotides
Ford S R, et al.
Journal of Bioluminescence and Chemiluminescence, 9(4), 251-265 (1994)
Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
Gels T, et al.
Analytical Biochemistry, 166(1), 368-379 (1987)
Tris-tricine and Tris-borate buffer systems provide better estimates of human mesothelial cell intermediate filament protein molecular weights than the standard Tris-glycine system
Patton W F, et al.
Analytical biochemistry, 197(1), 25-33 (1991)
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