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RAB0386

Sigma-Aldrich

Brain Natriuretic Peptide EIA Kit

for serum, plasma, culture supernatant and cell lysates

Synonym(s):

Gamma-brain natriuretic peptide, Natriuretic peptides B , BNP

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1 KIT
R 10 332,14

R 10 332,14

List PriceR 10 543,00

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1 KIT
R 10 332,14

About This Item

UNSPSC Code:
41123300
NACRES:
NA.32

R 10 332,14

List PriceR 10 543,00

Please contact Customer Service for Availability

species reactivity

human, mouse, rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
enzyme immunoassay: suitable

input

sample type serum
sample type culture supernatant(s)
sample type plasma
sample type cell lysate

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.66 pg/mL
standard curve range: 0.1–1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... NPPB(4879)

General description

The BNP Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting BNP peptide based on the principle of Competitive Enzyme Immunoassay.

Immunogen

Brain Natriuretic Peptide, synthetic peptide

Application

For research use only. Not for use in diagnostic procedures.
Brain natriuretic peptide EIA Kit has been used to measure brain natriuretic peptide (BNP) secreted in human neonatal ventricular cardiomyocyte culture.[1][2]

Biochem/physiol Actions

Brain natriuretic peptide (BNP) expressed in mammalian brain and atrial natriuretic peptide (ANP) facilitates various physiological functions including regulation of blood pressure and cardiac remodeling.[3] BNP acts as a potential biomarker of high left ventricular end-diastolic pressure in patients with symptomatic left ventricular (LV) dysfunction.[4] Elevated expression of BNP is observed in acute myocardial infarction (AMI) patients. BNP is also implicated in several biological functions such as diuresis, natriureis, hypotensive action and inhibition of aldosterone secretion.[3]

Kit Components Also Available Separately

Product No.
Description
SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Plasma brain natriuretic peptide as a biochemical marker of high left ventricular end-diastolic pressure in patients with symptomatic left ventricular dysfunction.
Maeda K
American Heart Journal, 135(5 pt 1), 825-832 (1998)
A new natriuretic peptide in porcine brain.
Sudoh T
Nature, 332(6159), 78-81 (1988)
Distinct T cell signatures define subsets of patients with multiple sclerosis.
Johnson MC
Neurology? neuroimmunology & neuroinflammation, 3(5), e278-e278 (2016)
Jiakan Weng et al.
Journal of cellular and molecular medicine, 23(2), 1050-1058 (2018-11-20)
Obesity is associated with increased cardiovascular morbidity and mortality, but the direct signals to initiate or exaggerate cardiomyopathy remain largely unknown. Present study aims to explore the pathophysiological role of autotaxin/lysophosphatidic acid (LPA) in the process of cardiomyopathy during obesity.
Yanbo Tang et al.
PloS one, 16(9), e0257280-e0257280 (2021-09-21)
Ulcerative colitis and Crohn's disease are classified as chronic inflammatory bowel diseases (IBD) with known extraintestinal manifestations. The interplay between heart and gut in IBD has previously been noted, but the mechanisms remain elusive. Our objective was to identify microRNAs

Questions

  1. What is the stability of the anti-BNP antibody concentrate (item N reconstituted with 55 ul of 1X Assay Diluent) from RAB0386 when stored at 4C?

    1 answer
    1. Storing and reusing the reconstituted antibody item N is not recommended as it is not stable when stored. The kit includes 2 vials so that a partial plate may be run.

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