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17173

Millipore

Rappaport Vassiliadis Broth, modified

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Magnesium chloride Malachite Green Broth, Modified Rappaport Vassiliadis Broth, RV Broth, Salmonella Enrichment Broth acc. to Rappaport and Vassiliadis

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500 G
R 2 189,32

R 2 189,32

List priceR 2 234,00
Please contact Customer Service for Availability
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500 G
R 2 189,32

About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

R 2 189,32

List priceR 2 234,00
Please contact Customer Service for Availability
Request a Bulk OrderRequest more information

Agency

according to ISO 19250:2010
according to ISO 6579-1:2017

Quality Level

200

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.2±0.2 (25 °C)

application(s)

environmental
food and beverages
food and beverages
pathogen testing
microbiology

suitability

selective for Salmonella spp.

Related Categories

Application

Used as a selective enrichment medium for the isolation of Salmonella species from food and environmental specimens.

Components

Papaic digest of soyabean meal 5 g/1110 mL;
Sodium chloride 8 g/1110 mL;
Monopotassium phosphate 1.6 g/1110 mL;
Magnesium chloride anhydrous 18.7 g/l (is equivalent to 40 g/1110 mL Magnesium chloride hexahydrate);
Malachite Green 0.04 g/1110 ml

Preparation Note

Suspend 30.0 g in 1 litre distilled water. Heat gently if necessary to dissolve the medium completely. Dispense as desired into tubes and sterilize by autoclaving at 115°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCM8972
BCCK9359
BCCK0802
BCCK2994
BCCJ0009

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Articles

Differentiation and ID Media for Salmonella

Salmonella, with 2,300+ serotypes, causes half of food-borne illnesses, often from dairy, poultry, and eggs.

Questions

  1. How to validate RV broth recovery and efficiency if autoclaved at 121

    1 answer

    1. The Rappaport Vassiliadis Broth with catalog no. 17173 has been validated for growth when autoclaved at 115 °C and not 121 °C for 15 minutes. We cannot confirm if the medium will perform as stated in the specification sheet available on our website if a different autoclaving temperature is applied as the medium is heat-sensitive.

      Should you wish to perform a validation of the performance of the medium after autoclaving it at 121°C, I would recommend performing growth promotion tests as listed in the datasheet to check for growth of the different strains listed therein.

      Helpful?

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