Protecting media from exposure to light has been a long-standing practice in maximizing the shelf-life. This is a common practice with both microbial and tissue culture media. These products include multiple components, some of which are undefined. While the specific reaction taking place cannot be provided, the more sensitive components of this formulation include the sodium thiosulfate, the sodium bisulfite, and the lecitihin. Please review the literature for each of these for specific reactions to extended exposure to light. The link below may be of interest:
https://jcp.bmj.com/content/jclinpath/22/3/273.full.pdf
D3435
Dey-Engley neutralizing broth
suitable for microbiology, NutriSelect® Plus
Synonym(s):
D/E Neutralizing Broth
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500 G
R 4 906,86
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500 G
R 4 906,86
About This Item
UNSPSC Code:
41171607
NACRES:
NA.85
Recommended Products
sterility
non-sterile
Quality Level
form
powder
shelf life
limited shelf life, expiry date on the label
manufacturer/tradename
NutriSelect® Plus
final pH
7.6±0.2 (25 °C)
application(s)
environmental
food and beverages
pharmaceutical
sterility testing
surface monitoring
microbiology
storage temp.
2-8°C
suitability
nonselective for Bacillus spp.
nonselective for Escherichia coli
nonselective for Pseudomonas spp.
nonselective for Salmonella spp.
nonselective for Staphylococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms
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General description
Dey-Engley Neutralizing Broth is used in disinfectant testing where the neutralization of the antiseptics and disinfectants is important for determining its bactericidal activity. The broth consists of tryptone that provides nitrogen and carbon sources, long chain amino acids, vitamins, and other essential nutrients. Dextrose is an energy source. Yeast extract is also a rich source of vitamin B-complex. Sodium bisulfite neutralizes aldehydes; sodium thioglycolate neutralizes mercurial; sodium thiosulfate neutralizes iodine and chlorine; lecithin neutralizes quaternary ammonium compounds; and polysorbate 80, a non-ionic surface-active agent, neutralizes substituted phenolics. Bromocresol purple is an indicator for dextrose utilization. Those organisms that ferment dextrose will turn the medium from purple to yellow. Growth of Pseudomonas sp., which does not ferment dextrose, can be detected by the formation of a pellicle on the surface of the broth.
Application
Dey-Engley neutralizing broth has been used to premoisten the sponge swab samples[1] to neutralise the residual action of the disinfectants on the microbiological growth.[2] Dey-Engley Neutralizing Broth is used in disinfectant testing where neutralization of the antiseptics and disinfectants for determining bactericidal activity. It has also been used to resuspend the Vermamoeba (Hartmannella) vermiformis cysts to neutralize peracetic acid (PAA).[3]
Dey-Engley Neutralizing Broth is used in disinfectant testing where neutralization of the antiseptics and disinfectants is important for determining bactericidal activity.
Biochem/physiol Actions
The Dey-Engley Neutralizing Broth neutralizes a broad spectrum of antiseptics and disinfectants including, quaternary ammonium compounds, phenolics, iodine, chlorine, mercurials, formaldehyde and glutaraldehyde. Sodium thioglycollate, sodium thiosulphate, sodium bisulphite, soya lecithin and polysorbate 80 act as neutralizing components. Growth is indicated by a color change from purple to yellow.
Components
Ingredients(g/L)
Casein enzymatic hydrolysate5.00
Yeast extract2.50
Dextrose10.00
Sodium thiosulfate6.00
Sodium thioglycollate1.00
Sodium bisulfite2.50
Lecithin7.00
Polysorbate 805.00
Bromocresol purple0.02
Casein enzymatic hydrolysate5.00
Yeast extract2.50
Dextrose10.00
Sodium thiosulfate6.00
Sodium thioglycollate1.00
Sodium bisulfite2.50
Lecithin7.00
Polysorbate 805.00
Bromocresol purple0.02
Preparation Note
Suspend 39 g of Dey-Engley Neutralizing Broth in 1000 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving 15 lbs. pressure (121°C) for 15 minutes.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Hi, please can you tell the technical reason for storing the prepared medium away from light...is there a specific component in the recipe that is light sensitive?
1 answer-
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I left the D/E dehydrated powder at room temperature for 2.5 months by mistake. Is the powder still okay to use, or should I get a replacement?
1 answer-
The powder may need to be replaced. The stability of this product stored in room temperature for 2.5 months can not validated. It would have to be determined by end-user.
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Hello. I would like to know at what temperature Dey Engley broth should be stored after it has been prepared and autoclaved.
1 answer-
Store prepared media below 8°C, protected from direct light. For more information please see the product information sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/373/551/d3435dat.pdf.
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