QBD10374

SPDP-dPEG^®₄-NHS ester

• Synonym(s): 2,5-Dioxo-1-pyrrolidinyl 17-oxo-19-(2-pyridinyldithio)-4,7,10,13-tetraoxa-16-azanonadecanoate
• Empirical Formula (Hill Notation): C₂₃H₃₃N₃O₉S₂
• CAS Number: 1334177-95-5
• Molecular Weight: 559.65
• MDL number: MFCD13185001
• UNSPSC Code: 12352108
• PubChem Substance ID: 329823921
• NACRES: NA.22

Properties

• Assay: >90%
• form: solid or viscous liquid
• reaction suitability: reactivity: thiol reactive; reagent type: cross-linking reagent; reactivity: amine reactive
• functional group: NHS ester
• polymer architecture: shape: linear; functionality: heterobifunctional
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: O=C(NCCOCCOCCOCCOCCC(ON1C(CCC1=O)=O)=O)CCSSC2=NC=CC=C2
• InChI: 1S/C23H33N3O9S2/c27-19(7-18-36-37-20-3-1-2-8-25-20)24-9-11-32-13-15-34-17-16-33-14-12-31-10-6-23(30)35-26-21(28)4-5-22(26)29/h1-3,8H,4-7,9-18H2,(H,24,27)
• InChI key: QTVBGVZVUCIFAH-UHFFFAOYSA-N

Description

Features and Benefits

SPDP-dPEG^®₄-NHS ester has an amine-reactive N-hydroxysuccinimide (NHS) ester at one end and a sulfhydryl-reactive 2-pyridyldithiol group at the opposite end. This has a single molecular weight PEG spacer arm, which confers greater water solubility to the crosslinker and linked proteins compared to crosslinkers having only hydrocarbon spacers. Protein aggregation and precipitation also are greatly reduced with SPDP-dPEG crosslinkers compared to crosslinkers that have only hydrocarbon spacers. dPEG spacer of specific length (23.1 Å) provides precise distance control between the SPDP moiety and the target molecule. Pyridyldithiol reagents produce disulfide-containing linkages that can be cleaved with reducing agents such as dithiothreitol (DTT). Crosslinking experiments with SPDP reagents are not limited to those involving proteins. Any of a variety of molecules with primary amines and sulfhydryl groups can be modified or crosslinked using SPDP reagents.

Legal Information

Products Protected under U.S. Patents # 7,888,536 B2

dPEG is a registered trademark of Quanta BioDesign

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable