SRP5348
GST protein, tag-free
recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
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About This Item
Produits recommandés
Produit recombinant
expressed in E. coli
Pureté
≥70% (SDS-PAGE)
Forme
buffered aqueous glycerol solution
Poids mol.
~25 kDa
Technique(s)
ligand binding assay: suitable
Numéro d'accès NCBI
Conditions d'expédition
dry ice
Température de stockage
−70°C
Informations sur le gène
Escherichia coli IAI39 ... gst> gst
gst(7152360)
Description générale
Glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial and microsomal proteins that are used to create the so-called GST gene fusion system. These proteins are capable of multiple reactions with a multitude of substrates. They have a role in detoxication and toxification mechanisms. GST tag has the size of 220 amino acids, which is relatively large compared to other tags like the myc- or the FLAG-tag that are quite small. GST can be used to purify and detect proteins of interest. In a GST gene fusion system, an expression vector consists of the gene sequence encoding the protein of interest and GST sequence alongside. After induction, the fusion proteins can be purified using affinity chromatography via its high affinity for glutathione.
Application
GST protein, tag-free has been used in in vitro F-actin binding assay. It has also been used as a protein tag for TPX2 (targeting protein for Xklp2).
Forme physique
Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Notes préparatoires
After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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