SRP3263

G-CSF human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonyme(s) : CSF-3, GM-CSF?, MGI-1G, pluripoietin
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Pureté: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Forme: lyophilized
• Puissance: 10.0-25.0 ng/mL
• Poids mol.: 18.7 kDa
• Conditionnement: pkg of 10 μg
• Impuretés: <0.1 EU/μg endotoxin, tested
• Couleur: white to off-white
• Numéro d'accès UniProt: P09919
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... CSF3(1440)

Description

Description générale

Granulocyte colony stimulating factor (G-CSF) or colony stimulating factor 3 (CSF-3) is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. Human and murine G-CSF are cross-species reactive. The gene encoding G-CSF is localized on human chromosome 17. Recombinant human G-CSF is an 18.7kDa protein consisting of 174 amino acid residues.

Actions biochimiques/physiologiques

Granulocyte colony stimulating factor (G-CSF) or colony stimulating factor 3 (CSF-3) is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It controls the production, differentiation and function of granulocytes. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. The protein has been shown to have a role in bone repair in animal models and clinically approved for neutropenia treatment.

Forme physique

Lyophilized from 5 mM Sodium Citrate, pH 4.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable