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SRE0111

Sigma-Aldrich

Heat-labile Cod Uracil-DNA Glycosylase

recombinant, expressed in E. coli, Triton-free

Synonyme(s) :

UDG, UNG

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About This Item

Code UNSPSC :
41106300
Nomenclature NACRES :
NA.54

Produit recombinant

expressed in E. coli

Niveau de qualité

200

Composition

Triton-free

Conditionnement

pkg of 100 U
pkg of 1000 U

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Uracil-DNA glycosylases are DNA repair enzymes that participate in the base excision repair pathway. Uracil DNA Glycosylase, also known as UNGor UDG, is an enzyme that hydrolyses the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA leaving an abasic(apyrimidinic) site in DNA. Contamination of PCR samples with amplicon DNA generated by previous reactions, “carry-over contamination”, is one of the major sources of false positive results. Heat-labile Cod Uracil-DNA Glycosylase prevents carry-over contamination in PCR, qPCR, RT-qPCR, and RT-LAMP assays. The enzyme is produced in a recombinant E. coli (ung–) strain that contains a modified Cod UNGgene.

Application

Heat-labile Cod Uracil-DNA Glycosylase eliminates carry-over contamination in PCR, RT-PCR, RT-qPCR, qPCR and RT-LAMP

Caractéristiques et avantages

  • Heat inactivation: The only UNG that is completely and irreversibly heat inactivated thus ensuring long-term sample integrity for subsequent post-PCR analysis such as cloning, sequencing or genotyping
  • Cod UNG is not active on uracil in RNA.
  • High sensitivity: Remove more than 108 copies of contaminating DNA without affecting the sensitivity (Cq) of the assay
  • Simple protocol: Applying Cod UNG in a RT-PCR is done by a 5 min Cod UNG preincubation step at RT prior to the amplification reaction

Définition de l'unité

One Unit is defined as the amount of enzyme that liberates 1 nmol Uracil per hour from Uracil-labelled DNA at 37 °C in 70 mm Tris-HCI pH 8.0 (@25 °C), 10 mM NaCI, 1 mM EDTA and 0.1 mg/ml BSA.

Reconstitution

In solution

Autres remarques

This product is for R&D use only. Not for drug, household, or other uses.

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2′-Deoxyadenosine 5′-triphosphate sodium salt solution, 100 mM, pH 7

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M-MLV Reverse Transcriptase, Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

W4502

Eau, Nuclease-Free Water, for Molecular Biology

D4184

Taq ADN polymérase JumpStart™, without MgCl2

DNTP100

Ensemble de désoxynucléotides, 100 mM, Individual dNTPs for routine PCR; 0.25 mL each

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Quantitative RT-PCR ReadyMix™, One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

UDGHL-RO

Uracile-ADN glycosylase thermolabile, recombinant from marine bacterium BMTU 3346

MBD0025

Water, Microbial DNA-free; Suitable for PCR, sequencing and NGS

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Uracil-DNA glycosylases?Structural and functional perspectives on an essential family of DNA repair enzymes
N Schormann et al.
Protein Science, 1667?1685-1667?1685 (2014)

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