SAE0092
Mutanolysine from Streptomyces globisporus ATCC 21553
free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)
Synonyme(s) :
Mutanolysin
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About This Item
Produits recommandés
Source biologique
Streptomyces sp. (Streptomyces globisporus ATCC 21553)
Forme
lyophilized powder
Activité spécifique
≥4000 units/mg protein (biuret)
Poids mol.
23 kDa
~18.5 kDa
Caractéristiques
DNA free
Concentration
≥40% protein
Technique(s)
nucleic acid detection: suitable
Adéquation
suitable for cell lysis
Application(s)
life science and biopharma
Conditions d'expédition
wet ice
Température de stockage
−20°C
Application
Elle permet de réaliser une lyse douce pour isoler les biomolécules facilement dégradables et l′ARN de bactéries. Elle a servi à la formation de sphéroplastes pour isoler l′ADN.
Purified Mutanolysin SAE0092 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Mutanolysin is a muralytic enzyme, suitable for usage in isolation of nucleic acids, from Gram-positive bacteria (e.g. Listeria, Lactobacillus, Lactococcus),(8) and also from other bacteria that are difficult to lyse with lysozyme only.
Mutanolysin is a muralytic enzyme, suitable for usage in isolation of nucleic acids, from Gram-positive bacteria (e.g. Listeria, Lactobacillus, Lactococcus),(8) and also from other bacteria that are difficult to lyse with lysozyme only.
Actions biochimiques/physiologiques
La mutanolysine est une N-acétylmuramidase. Comme le lysozyme, il s′agit d′une enzyme muralytique qui clive la liaison de la β-N-acétylmuramyl-(1→4)-N-acétylglucosamine du polymère peptidoglycane-polysaccharide des parois cellulaires bactériennes. Ses extrémités carboxyliques jouent un rôle dans la reconnaissance et la liaison de polymères uniques des parois cellulaires. La mutanolysine lyse les bactéries Listeria et autres bactéries à Gram positif comme Lactobacillus et Lactococcus.
Caractéristiques et avantages
Suitable for difficult-to-lyse samples.
Microbial DNA free, minimizing DNA contamination from reagents in workflow.
Suitable for the isolation of nucleic acids from Gram-positive bacteria, including Listeria, Lactobacillus, and Lactococcus.
Microbial DNA free, minimizing DNA contamination from reagents in workflow.
Suitable for the isolation of nucleic acids from Gram-positive bacteria, including Listeria, Lactobacillus, and Lactococcus.
Définition de l'unité
One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.
Forme physique
Lyophilized powder containing Ficoll® and sodium succinate buffer salts
Informations légales
Ficoll is a registered trademark of Cytiva
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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