MAK311
Peroxide Assay Kit
sufficient for 250 colorimetric tests
Synonyme(s) :
Hydrogen Peroxide Test Kit
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Méthode de détection
colorimetric
Maladie(s) pertinente(s)
cardiovascular diseases; aging/geriatric diseases; neonatal diseases; pediatric diseases; orthopedic diseases; neurological disorders; pulmonary disorders
Température de stockage
2-8°C
Catégories apparentées
Description générale
Peroxide (e.g. hydrogen peroxide H2O2), a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.
Application
Peroxide Assay Kit has been used to measure hydrogen peroxide level in rat tissue homogenates.
Caractéristiques et avantages
Compatible with high-throughput handling systems.
Adéquation
Suitable for the quantitative determination of peroxide concentration without any pretreatment in a variety of biological samples, such as serum, citrate-plasma, urine, cell lysates, and culture medium.
Principe
The Peroxide Assay Kit provides a simple and high-throughput adaptable assay for quantitative determination of peroxide concentration without any pretreatment in a variety of biological samples, such as serum, citrate-plasma, urine, cell lysates, and culture medium. This assay utilizes the chromogenic Fe3+ −xylenol orange reaction, in which a purple complex is formed when Fe2+ is oxidized to Fe3+ by peroxides present in the sample, generating a colorimetric (585 nm) result, proportional to the level of peroxide present. The optimized formulation reduces interference by substances in the raw samples. A concentration of 1 μM H2O2 equals 34 ng/mL or 34 ppb. The kit has a detection range of 0.2–30 μM (7–1,020 ng/mL) H2O2 in a 96 well format.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
Code de la classe de stockage
8B - Non-combustible corrosive hazardous materials
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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